Product Details
| MW: | ~26kDa. |
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| Source: | Produced in E. coli. Active human granzyme K. |
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| EC: | 3.4.21.- |
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| UniProt ID: | P49863 |
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| Formulation: | Liquid. In PBS containing 10% sucrose and ~0.5M sodium chloride. Contains no preservatives. |
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| Purity: | ≥98% (SDS-PAGE) |
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| Specific Activity: | One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25ºC in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB. Specific activity provided in lot specific CoA. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least 6 months when stored at -80°C. Dilute solutions (e. g. 1-1000ng/ml) should be used within 24 hours. |
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| Handling: | For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -80°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma: E. Wilharm, et al.; J. Biol. Chem.
274, 27331 (1999),
Abstract;
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