Product Details
| Alternative Name: | ICH-2, Protease TX, ICErel-II |
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| Source: | Produced in E. coli. Contains an N-terminal His-tag. |
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| EC: | 3.4.22.57 |
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| UniProt ID: | P49662 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Specific Activity: | ~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT. |
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| Application Notes: | Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity. |
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| Reconstitution: | Reconstitute with PBS containing 15% glycerol to 1U/µl. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. |
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| Scientific Background: | Caspase-4 is a member of the caspase family of cysteine proteases. It exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits characteristic of the native enzyme. The active caspase-4 is routinely tested for its ability to enzymatically cleave the substrate WEHD-AFC or WEHD-pNA. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
MDA-7/IL-24 potently induces apoptosis in human myeloid leukemia cells through a process regulated by ER stress: M. Rahmani, et al.; Mol. Pharmacol.
78, 1096 (2010),
Abstract;
Full Text
The pan-HDAC inhibitor vorinostat potentiates the activity of the proteasome inhibitor carfilzomib in human DLBCL cells in vitro and in vivo: G. Dasmahapatra, et al.; Blood
115, 4478 (2010),
Abstract;
Full Text
