Active form of human SUMO-2 for conjugation to protein substrates.
Product Details
| Alternative Name: | Sentrin-2, Small ubiquitin-related modifier 2, SUMO-3 |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P61956 |
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| Formulation: | Liquid. In PBS. |
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| Purity: | ≥95% |
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| Application Notes: | Standard reaction for protein conjugation (10µl) includes the target protein, SUMO-1 activating enzyme (Prod. No. BML-UW9330) (100ng), UbcH9 (900ng) (Prod. No. ALX-201-046), SUMO-2 (1-93) (10µg) in 50mM TRIS, pH 7.6, 5mM magnesium chloride, 2mM ATP, 10mM creatine phosphate, 3.5U/ml of creatine kinase and 0.6U/ml of inorganic pyrophosphatase. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for a few days at room temperature. |
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| Technical Info/Product Notes: | Formerly this product was called SUMO-3 (1-93) (human) (recombinant), but the name was changed in accordance with UniProt. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
An electrophoretic mobility shift assay identifies a mechanistically unique inhibitor of protein sumoylation: Y.S. Kim, et al.; Chem. Biol.
20, 604 (2013),
Abstract;
Full Text
Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9: M.H. Tatham, et al.; J. Biol. Chem.
276, 35368 (2001),
Abstract;
Full Text
Protein modification by SUMO: R.T. Hay; TIBS
26, 332 (2001),
Abstract;
Ubch9 conjugates SUMO but not ubiquitin: J.M. Desterro, et al.; FEBS Lett.
417, 297 (1997),
Abstract;
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