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SUMO-2 (human) (1-93), (recombinant)

Active form of SUMO-2
ALX-201-089-C500 500 µg 356.00 USD
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Active form of human SUMO-2 for conjugation to protein substrates.

Product Details

Alternative Name:Sentrin-2, Small ubiquitin-related modifier 2, SUMO-3
Source:Produced in E. coli.
UniProt ID:P61956
Formulation:Liquid. In PBS.
Application Notes:Standard reaction for protein conjugation (10µl) includes the target protein, SUMO-1 activating enzyme (Prod. No. BML-UW9330) (100ng), UbcH9 (900ng) (Prod. No. ALX-201-046), SUMO-2 (1-93) (10µg) in 50mM TRIS, pH 7.6, 5mM magnesium chloride, 2mM ATP, 10mM creatine phosphate, 3.5U/ml of creatine kinase and 0.6U/ml of inorganic pyrophosphatase.
Shipping:Blue Ice
Long Term Storage:-80°C
Use/Stability:Stable for a few days at room temperature.  
Technical Info/Product Notes:Formerly this product was called SUMO-3 (1-93) (human) (recombinant), but the name was changed in accordance with UniProt.
Regulatory Status:RUO - Research Use Only

Product Literature References

An electrophoretic mobility shift assay identifies a mechanistically unique inhibitor of protein sumoylation: Y.S. Kim, et al.; Chem. Biol. 20, 604 (2013), Abstract; Full Text
Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9: M.H. Tatham, et al.; J. Biol. Chem. 276, 35368 (2001), Abstract; Full Text
Protein modification by SUMO: R.T. Hay; TIBS 26, 332 (2001), Abstract;
Ubch9 conjugates SUMO but not ubiquitin: J.M. Desterro, et al.; FEBS Lett. 417, 297 (1997), Abstract;

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