| ALX-201-078-C005 | 5 µg | 399.00 USD |  |
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| Alternative Name: | CPP32, Yama, Apopain |
| Source: | Produced in E. coli. |
| EC: | 3.4.22.- |
| UniProt ID: | P55213 |
| Concentration: | 0.165mg/ml |
| Formulation: | Liquid. 5µg of caspase-3 in 50mM HEPES, pH 7.4, 100mM NaCI, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 50% glycerol. |
| Purity: | ≥95% (SDS-PAGE) |
| Specific Activity: | ≥500U/µg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase-substrate Z-DEVD-AMC per hour at 20°C in a reaction solution containing 20µM substrate, 50mM HEPES, pH 7.4, 100mM NaCl, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 10% glycerol. |
| Application Notes: | In combination with caspase-3 activity assays, this product is useful in biological screening of caspase substrates and inhibitors. It can also be used as a positive control in caspase-3 assays as well as a positive control in Western blot. |
| Shipping: | Dry Ice |
| Short Term Storage: | -20°C |
| Long Term Storage: | -80°C |
| Use/Stability: | Stability at -20°C: 1-2 weeks |
| Handling: | Avoid freeze/thaw cycles. |
| Protocol: | Enzyme Evaluation Protocol (general) This protocol can be used to measure caspase enzyme activity. A synthetic fluorogenic peptide, e. g. Ac-DEVD-AMC (Prod. No. ALX-260-031) is used as the caspase enzyme substrate. The enzyme cleaves the substrate releasing the fluorescent AMC. AMC release can be measured by spectrofluorometry using UV. 1. Add 20µM of Ac-DEVD-AMC to 1 ml of assay buffer (20mM PIPES, pH 7.2, 100mM sodium chloride, 10mM DTT, 1mM EDTA, 0.1% (w/v) CHAPS, 10% sucrose). Add the appropriate amount of active caspase to the mixture (e.g. 50ng/ml). 2. Incubate for 1 hour at 37°C. 3. Measure the AMC liberated from the Ac-DEVD-AMC using a spectrofluorometer with an excitation wavelength of 380nm and an emission wavelength of 440nm. |
| Regulatory Status: | RUO - Research Use Only |
Ac-DEVD-AMC |
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| Caspase substrate | ||||||
| ≥97% (HPLC) | Print as PDF | ||||||
| ALX-260-031-M001 | 1 mg | 71.00 USD | |
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| ALX-260-031-M005 | 5 mg | 199.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
