Product Details
| Alternative Name: | Mch3, CMH-1 |
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| Source: | Produced in E. coli. Contains a C-terminal His-tag. |
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| EC: | 3.4.22.60 |
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| UniProt ID: | P55210 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Specific Activity: | ~25’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-pNA (pNA: p-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT. |
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| Application Notes: | Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity. |
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| Reconstitution: | Reconstitute to 1U/µl with PBS containing 15% glycerol. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | After reconstitution, prepare aliquots and store at -80°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Characterization of circulating molecules and activities in plasma of patients after allogeneic and autologous intraoral bone grafting procedures: a prospective randomized controlled clinical trial in humans: Ö. Solakoğlu, et al.; BMC Oral Health
22, 24 (2022),
Abstract;
The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-9: J. Zhang, et al.; Cell Res.
31, 980 (2021),
Abstract;
Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis: B. Zhou, et al.; Cell Res.
28, 1171 (2018),
Abstract;
Full Text
Pyroptosis and apoptosis pathways engage in bidirectional crosstalk in monocytes and macrophages: C.Y. Taabazuing, et al.; Cell Chem. Biol.
24, 507 (2017),
Abstract;
Full Text
Avian reovirus-triggered apoptosis enhances both virus spread and the processing of the viral nonstructural muNS protein: J. Rodriguez-Grille, et al.; Virology
462, 49 (2014),
Abstract;
