Product Details
| Alternative Name: | CPP32, Yama, Apopain |
| |
| Source: | Produced in E. coli. Contains an N-terminal His-tag. |
| |
| EC: | 3.4.22.56 |
| |
| UniProt ID: | P42574 |
| |
| Formulation: | Lyophilized. |
| |
| Quality Control: | Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD-pNA (Prod. No. ALX-260-033) and Ac-DEVD-AFC (Prod. No. ALX-260-032). |
| |
| Specific Activity: | ≥15,000 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-pNA (Prod. No. ALX-260-033) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT. |
| |
| Application Notes: | Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No. ALX-850-216 or ALX-850-215). |
| |
| Reconstitution: | Reconstitute to 1U/µl in water. |
| |
| Shipping: | Dry Ice |
| |
| Long Term Storage: | -80°C |
| |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. |
| |
| Scientific Background: | Caspase-3 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. It exists in cells as an inactive 32kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and granzyme B. The processed form of caspase-3 consists of large (17kDa) and small (11kDa) subunits which associate to form the active enzyme. Active caspase-3 has been shown involved in the proteolysis of several important molecules, such as poly(ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK) and others. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Figure: Active human caspase was expressed in E. coli and purified. The activity of recombinant caspase-3 was determined by cleaving AFC conjugates of DEVD. The cleavage activity was effectively inhibited by the corresponding peptide inhibitor as indicated.
Please mouse over
Product Literature References
Conjunctival epitheliopathy induced by topical exposure to bacterial peptidoglycan, muramyl dipeptide: M.P. Langford, et al.; Exp. Eye Res.
227, 109383 (2023),
Abstract;
Cardioprotective Effects of Oroxylum indicum Extract Against Doxorubicin and Cyclophosphamide-Induced Cardiotoxicity: S.R. Pondugula, et al.; Cardiovasc. Toxicol.
22, 67 (2022),
Abstract;
Characterization of circulating molecules and activities in plasma of patients after allogeneic and autologous intraoral bone grafting procedures: a prospective randomized controlled clinical trial in humans: Ö. Solakoğlu, et al.; BMC Oral Health
22, 24 (2022),
Abstract;
GSDMEa-mediated pyroptosis is bi-directionally regulated by caspase and required for effective bacterial clearance in teleost: H. Xu, et al.; Cell Death Dis.
13, 491 (2022),
Abstract;
Aptamer-based enrichment of TDP-43 from human cells and tissues with quantification by HPLC-MS/MS: P.D. Taylor, et al.; J. Neurosci. Methods
361, 109344 (2021),
Abstract;
BAD sensitizes breast cancer cells to docetaxel with increased mitotic arrest and necroptosis: J. Mann, et al.; Sci. Rep.
10, 355 (2020),
Abstract;
Full Text
Caspase-Activated Oligonucleotide Probe: L. Yang, et al.; Bioconjug. Chem.
31, 2172 (2020),
Abstract;
Separase-triggered apoptosis enforces minimal length of mitosis: S. Hellmuth & O. Stemmann; Nature
580, 542 (2020),
Application(s): In vitro cleavage assay,
Abstract;
Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress: A. Shemorry, et al.; Elife
8, e47084 (2019),
Abstract;
Full Text
Abolishing Tau cleavage by caspases at Aspartate421 causes memory/synaptic plasticity deficits and pre-pathological Tau alterations: F. Biundo, et al.; Transl. Psychiatry
7, e1198 (2017),
Abstract;
Full Text
Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis: N. Rukoyatkina, et al.; Cell Death Dis.
8, e2898 (2017),
Abstract;
Full Text
BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation: K. Xie, et al.; Autophagy
12, 381 (2016),
Abstract;
Full Text
Avian reovirus-triggered apoptosis enhances both virus spread and the processing of the viral nonstructural muNS protein: J. Rodriguez-Grille, et al.; Virology
462, 49 (2014),
Abstract;
Leukocyte protease binding to nucleic acids promotes nuclear localization and cleavage of nucleic acid binding proteins: M.P. Thomas, et al.; J. Immunol.
192, 5390 (2014),
Abstract;
Structural order in Pannexin 1 cytoplasmic domains: G. Spagnol, et al.; Channels
8, 157 (2014),
Abstract;
Full Text
Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
300, R531 (2011),
Abstract;
Full Text
Related Products
