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Caspase-3 (human), (recombinant) (active)

Highly active caspase essential for apoptosis
ALX-201-059-U025 25 U 199.00 USD
ALX-201-059-U100 100 U 455.00 USD
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Product Details

Alternative Name:CPP32, Yama, Apopain
Source:Produced in E. coli. Contains an N-terminal His-tag.
UniProt ID:P42574
Quality Control:Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD-pNA (Prod. No. ALX-260-033) and Ac-DEVD-AFC (Prod. No. ALX-260-032).
Specific Activity:≥15,000 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-pNA (Prod. No. ALX-260-033) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Application Notes:Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No. ALX-850-216 or ALX-850-215).
Reconstitution:Reconstitute to 1U/µl in water.
Shipping:Dry Ice
Long Term Storage:-80°C
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Scientific Background:Caspase-3 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. It exists in cells as an inactive 32kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and granzyme B. The processed form of caspase-3 consists of large (17kDa) and small (11kDa) subunits which associate to form the active enzyme. Active caspase-3 has been shown involved in the proteolysis of several important molecules, such as poly(ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK) and others.
Regulatory Status:RUO - Research Use Only
Caspase-3 (human), (recombinant) (active) image
Figure: Active human caspase was expressed in E. coli  and purified. The activity of recombinant caspase-3 was determined by cleaving AFC conjugates of DEVD. The cleavage activity was effectively inhibited by the corresponding peptide inhibitor as indicated. 
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Caspase-3 (human), (recombinant) (active) image

Product Literature References

Conjunctival epitheliopathy induced by topical exposure to bacterial peptidoglycan, muramyl dipeptide: M.P. Langford, et al.; Exp. Eye Res. 227, 109383 (2023), Abstract;
Cardioprotective Effects of Oroxylum indicum Extract Against Doxorubicin and Cyclophosphamide-Induced Cardiotoxicity: S.R. Pondugula, et al.; Cardiovasc. Toxicol. 22, 67 (2022), Abstract;
Characterization of circulating molecules and activities in plasma of patients after allogeneic and autologous intraoral bone grafting procedures: a prospective randomized controlled clinical trial in humans: Ö. Solakoğlu, et al.; BMC Oral Health 22, 24 (2022), Abstract;
GSDMEa-mediated pyroptosis is bi-directionally regulated by caspase and required for effective bacterial clearance in teleost: H. Xu, et al.; Cell Death Dis. 13, 491 (2022), Abstract;
Aptamer-based enrichment of TDP-43 from human cells and tissues with quantification by HPLC-MS/MS: P.D. Taylor, et al.; J. Neurosci. Methods 361, 109344 (2021), Abstract;
BAD sensitizes breast cancer cells to docetaxel with increased mitotic arrest and necroptosis: J. Mann, et al.; Sci. Rep. 10, 355 (2020), Abstract; Full Text
Caspase-Activated Oligonucleotide Probe: L. Yang, et al.; Bioconjug. Chem. 31, 2172 (2020), Abstract;
Separase-triggered apoptosis enforces minimal length of mitosis: S. Hellmuth & O. Stemmann; Nature 580, 542 (2020), Application(s): In vitro cleavage assay, Abstract;
Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress: A. Shemorry, et al.; Elife 8, e47084 (2019), Abstract; Full Text
Abolishing Tau cleavage by caspases at Aspartate421 causes memory/synaptic plasticity deficits and pre-pathological Tau alterations: F. Biundo, et al.; Transl. Psychiatry 7, e1198 (2017), Abstract; Full Text
Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis: N. Rukoyatkina, et al.; Cell Death Dis. 8, e2898 (2017), Abstract; Full Text
BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation: K. Xie, et al.; Autophagy 12, 381 (2016), Abstract; Full Text
Avian reovirus-triggered apoptosis enhances both virus spread and the processing of the viral nonstructural muNS protein: J. Rodriguez-Grille, et al.; Virology 462, 49 (2014), Abstract;
Leukocyte protease binding to nucleic acids promotes nuclear localization and cleavage of nucleic acid binding proteins: M.P. Thomas, et al.; J. Immunol. 192, 5390 (2014), Abstract;
Structural order in Pannexin 1 cytoplasmic domains: G. Spagnol, et al.; Channels 8, 157 (2014), Abstract; Full Text
Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 300, R531 (2011), Abstract; Full Text

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