Product Details
Alternative Name: | CPP32, Yama, Apopain |
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Source: | Produced in E. coli. Contains an N-terminal His-tag. |
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EC: | 3.4.22.56 |
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UniProt ID: | P42574 |
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Formulation: | Lyophilized. |
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Quality Control: | Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD-pNA (Prod. No. ALX-260-033) and Ac-DEVD-AFC (Prod. No. ALX-260-032). |
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Specific Activity: | ≥15,000 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-pNA (Prod. No. ALX-260-033) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT. |
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Application Notes: | Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No. ALX-850-216 or ALX-850-215). |
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Reconstitution: | Reconstitute to 1U/µl in water. |
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Shipping: | Dry Ice |
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Long Term Storage: | -80°C |
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Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. |
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Scientific Background: | Caspase-3 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. It exists in cells as an inactive 32kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and granzyme B. The processed form of caspase-3 consists of large (17kDa) and small (11kDa) subunits which associate to form the active enzyme. Active caspase-3 has been shown involved in the proteolysis of several important molecules, such as poly(ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK) and others. |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Active human caspase was expressed in E. coli and purified. The activity of recombinant caspase-3 was determined by cleaving AFC conjugates of DEVD. The cleavage activity was effectively inhibited by the corresponding peptide inhibitor as indicated.
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Product Literature References
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Abolishing Tau cleavage by caspases at Aspartate421 causes memory/synaptic plasticity deficits and pre-pathological Tau alterations: F. Biundo, et al.; Transl. Psychiatry
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