Product Details
| Alternative Name: | Interleukin 1β converting enzyme, IL-1β converting enzyme, ICE |
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| Source: | Produced in E. coli. Contains an N-terminal His-tag. |
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| EC: | 3.4.22.36 |
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| UniProt ID: | P29466 |
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| Formulation: | Lyophilized. |
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| Quality Control: | Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-pNA (Prod. No. ALX-260-026). |
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| Specific Activity: | >5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-pNA (pNA: p-nitroaniline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT. |
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| Application Notes: | Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity. |
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| Reconstitution: | Reconstitute to 1U/µl in water |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. |
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| Scientific Background: | Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
GSDMEa-mediated pyroptosis is bi-directionally regulated by caspase and required for effective bacterial clearance in teleost: H. Xu, et al.; Cell Death Dis.
13, 491 (2022),
Abstract;
The colonic pathogen Entamoeba histolytica activates caspase-4/1 that cleaves the pore-forming protein gasdermin D to regulate IL-1β secretion: S. Wang, et al.; PLoS Pathog.
18, e1010415 (2022),
Abstract;
Entamoeba histolytica activation of caspase-1 degrades cullin that attenuates NF-κB dependent signaling from macrophages: A. Chadha, et al.; PLoS Pathog.
17, 1009936 (2021),
Abstract;
The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-10: J. Zhang, et al.; Cell Res.
31, 980 (2021),
Abstract;
An innate interaction between IL-18 and the propeptide that inactivates its precursor form: N. Tsutnami, et al.; Sci. Rep.
9, 6160 (2019),
Abstract;
Full Text
Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis: B. Zhou, et al.; Cell Res.
28, 1171 (2018),
Abstract;
Full Text
Syk is involved in NLRP3 inflammasome-mediated caspase-1 activation through adaptor ASC phosphorylation and enhanced oligomerization: Y.C. Lin, et al.; J. Leukoc. Biol.
97, 825 (2015),
Abstract;
Interleukin-33 Is Biologically Active Independently of Caspase-1 Cleavage: D. Talabot-Ayer, et al.; J. Biol. Chem.
284, 19420 (2009),
Abstract;
Full Text
C/EBPbeta phosphorylation by RSK creates a functional XEXD caspase inhibitory box critical for cell survival: M. Buck, et al.; Mol. Cell
8, 807 (2001),
Abstract;
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