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Caspase-1 (human), (recombinant) (active)

ALX-201-056-U025 25 U 392.00 USD
ALX-201-056-U100 100 U 658.00 USD
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Product Details

Alternative Name:Interleukin 1β converting enzyme, IL-1β converting enzyme, ICE
Source:Produced in E. coli. Contains an N-terminal His-tag.
UniProt ID:P29466
Quality Control:Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-pNA (Prod. No. ALX-260-026).
Specific Activity:>5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-pNA (pNA: p-nitroaniline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Application Notes:Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
Reconstitution:Reconstitute to 1U/µl in water
Shipping:Dry Ice
Long Term Storage:-80°C
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Scientific Background:Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
Regulatory Status:RUO - Research Use Only

Product Literature References

GSDMEa-mediated pyroptosis is bi-directionally regulated by caspase and required for effective bacterial clearance in teleost: H. Xu, et al.; Cell Death Dis. 13, 491 (2022), Abstract;
The colonic pathogen Entamoeba histolytica activates caspase-4/1 that cleaves the pore-forming protein gasdermin D to regulate IL-1β secretion: S. Wang, et al.; PLoS Pathog. 18, e1010415 (2022), Abstract;
Entamoeba histolytica activation of caspase-1 degrades cullin that attenuates NF-κB dependent signaling from macrophages: A. Chadha, et al.; PLoS Pathog. 17, 1009936 (2021), Abstract;
The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-10: J. Zhang, et al.; Cell Res. 31, 980 (2021), Abstract;
An innate interaction between IL-18 and the propeptide that inactivates its precursor form: N. Tsutnami, et al.; Sci. Rep. 9, 6160 (2019), Abstract; Full Text
Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis: B. Zhou, et al.; Cell Res. 28, 1171 (2018), Abstract; Full Text
Syk is involved in NLRP3 inflammasome-mediated caspase-1 activation through adaptor ASC phosphorylation and enhanced oligomerization: Y.C. Lin, et al.; J. Leukoc. Biol. 97, 825 (2015), Abstract;
Interleukin-33 Is Biologically Active Independently of Caspase-1 Cleavage: D. Talabot-Ayer, et al.; J. Biol. Chem. 284, 19420 (2009), Abstract; Full Text
C/EBPbeta phosphorylation by RSK creates a functional XEXD caspase inhibitory box critical for cell survival: M. Buck, et al.; Mol. Cell 8, 807 (2001), Abstract;

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