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Ubc9 (human), (recombinant)

Conjugating enzyme (E2)
ALX-201-046-C050 50 µg 258.00 USD
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Product Details

Source:Produced in E. coli.
UniProt ID:P63279
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 5mM β-mercaptoethanol.
Application Notes:Ubc9 catalyzes formation of an isopeptide bond between the C-terminus of SUMO-1/sentrin-1 and ε-amino groups in the target protein.

Standard reaction for protein conjugation (10µl) includes the target protein, SUMO-1 activating enzyme (Prod. No. BML-UW9330) (100ng), Ubc9 (900ng) (Prod. No. ALX-201-046), SUMO-1 (1-97) (Prod. No. ALX-201-045) (10µg) in 50mM TRIS, pH 7.6, 5mM magnesium chloride, 2mM ATP, 10mM creatine phosphate, 3.5U/ml of creatine kinase and 0.6U/ml of inorganic pyrophosphatase.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Regulatory Status:RUO - Research Use Only

Product Literature References

SUMO-1 modification activates the transcriptional response of p53: M.S. Rodriguez, et al.; EMBO J. 18, 6455 (1999), Abstract; Full Text
SUMO-1 modification of IkappaBalpha inhibits NF-kappaB activation: J.M. Desterro, et al.; Mol. Cell 2, 233 (1998), Abstract;
Ubch9 conjugates SUMO but not ubiquitin: J.M. Desterro, et al.; FEBS Lett. 417, 297 (1997), Abstract;

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