Product Details
| Alternative Name: | OB gene product |
| |
| MW: | ~16kDa. |
| |
| Source: | Produced in E. coli. Homologous to mouse serum leptin. |
| |
| CAS: | 181030-10-4 |
| |
| MI: | 14: 5443 |
| |
| UniProt ID: | P41160 |
| |
| Formulation: | Lyophilized from 0.0045mM sodium bicarbonate. |
| |
| Purity: | ≥98% (SDS-PAGE) |
| |
| Endotoxin Content: | <0.1EU/µg purified protein. |
| |
| Biological Activity: | Induces proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |
| |
| Reconstitution: | Reconstitute with sterile water or 0.4% sodium bicarbonate, pH 8-9. Do not reconstitute to less than 0.1mg/ml. Further dilutions should be made with medium containing a carrier protein. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | Reconstituted protein is stable for 4 weeks when stored at +4°C. |
| |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| |
| Scientific Background: | Leptin, the product of the ob (obese) gene, is a 16kDa protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. Studies have shown that it may also influence reproductive function. Mice with ob/ob genotype develop a form of diabetes similar to type II in humans. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
General Literature References
Effects of the obese gene product on body weight regulation in ob/ob mice: M.A. Pelleymounter, et al.; Science
269, 540 (1995),
Abstract;
Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects: M. Maffei, et al.; Nature Med.
1, 1155 (1995),
Abstract;
Weight-reducing effects of the plasma protein encoded by the obese gene: J.L. Halaas, et al.; Science
269, 543 (1995),
Abstract;
Positional cloning of the mouse obese gene and its human homologue: Y. Zhang, et al.; Nature
372, 425 (1994),
Abstract;
Related Products
