Does not activate TLR2. Can be used as negative control for Pam3Cys-Ser-(Lys)4 . trihydrochloride (Prod. No. ALX-165-066).
Product Details
| Alternative Name: | PHCSK4 . 3 TFA, N-palmitoyl-S-(1,2-bishexadecyloxy-carbonyl)-ethyl-(R)-cysteinyl-seryl-(lysyl)3-lysine . 3 CF3COOH |
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| Formula: | C82H158N10O13S . 3 CF3COOH |
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| MW: | 1524.3 . 342.1 |
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| Source: | Synthetic |
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| Concentration: | 1mg/ml after reconstitution. |
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| Formulation: | Lyophilized. |
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| Quality Control: | Confirmed by ESI-MS. |
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| Appearance: | Colorless powder |
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| Solubility: | Soluble in pyrogen-free deionized water, DMSO or aqueous buffers at pH <7.5 |
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| Reconstitution: | Reconstitute with 1 mL pyrogen-free deionized water in a pyrogen-free tube. Reconstitution can also be performed in DMSO (1mg/ml stock solution). Homogenize by vortexing or use a homogenizer or sonicator to prepare a homogenous solution. Further dilutions can be made with cell culture medium or pyrogen-free deionized water. PBS is not recommended for reconstitution or dilution. |
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| Shipping: | Ambient |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Lipopeptides are valuable tools for basic research in innate and acquired immunity. PHC-SKKKK (PHC-SK4) and Pam3Cys-SKKKK (P3SK4) (Prod. No. ALX-165-066) have similar physicochemical properties, both have three fatty acid residues and the same peptide moiety. In contrast to P3SK4, PHCSK4 is not an activator of TLR2. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The synthetic bacterial lipopeptide Pam3CSK4 modulates respiratory syncytial virus infection independent of TLR activation: D.T. Nguyen, et al.; PLoS Pathog.
6, e1001049 (2010),
Abstract;
Synthetic bacterial lipopeptide analogs: structural requirements for adjuvanticity: M. Ghielmetti, et al.; Immunobiology
210, 211 (2005),
Abstract;
Antagonistic antibody prevents toll-like receptor 2-driven lethal shock-like syndromes: G. Meng, et al.; J. Clin. Invest.
113, 1473 (2004),
Abstract;
The ester-bonded palmitoyl side chains of Pam3CysSerLys4 lipopeptide account for its powerful adjuvanticity to HLA class I-restricted CD8+ T lymphocytes: A. Reschner, et al.; Eur. J. Immunol.
33, 2044 (2003),
Abstract;
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