Replaces Prod. #: BML-P117
Excellent substrate for protein kinase C (PKC) (Km = 20nM for purified rat brain PKC). The peptide consists of residues 151-175 of the myristoylated alanine-rich C-kinase substrate (MARCKS) protein phosphorylation site. Protein kinase C isotypes (including PKCε) phosphorylate serines 8, 12 and 19 of this peptide. It is a very weak substrate for cAMP- and cGMP-dependent protein kinase and CaM kinases I, II and III.
Product Details
| Sequence: | H-Lys-Lys-Lys-Lys-Lys-Arg-Phe-Ser-Phe-Lys-Lys-Ser-Phe-Lys-Leu-Ser-Gly-Phe-Ser-Phe-Lys-Lys-Asn-Lys-Lys-OH |
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| Formula: | C147H243N41O31 |
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| MW: | 3081.8 |
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| CAS: | 125267-21-2 |
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| Formulation: | Lyophilized |
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| Purity: | ≥98% |
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| Appearance: | White to off-white powder. |
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| Solubility: | Soluble in water. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The MARCKS family of cellular protein kinase C substrates: P.J. Blackshear; J. Biol. Chem.
268, 1501 (1993), (Minireview),
Abstract;
Full Text
Protein kinase C substrate and inhibitor characteristics of peptides derived from the myristoylated alanine-rich C kinase substrate (MARCKS) protein phosphorylation site domain: J.M. Graff, et al.; J. Biol. Chem.
266, 14390 (1991),
Abstract;
Full Text
General Literature References
Multisite phosphorylation of the 80 kDa (MARCKS) protein kinase C substrate in C3H/10T1/2 fibroblasts. Quantitative analysis of individual sites by solid-phase microsequencing.: B. Amess et al.; FEBS Lett.
297, 285 (1992),
Abstract;
