Product Details
| Alternative Name: | Synaptobrevin, Vesicle-associated membrane protein 2 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to a portion of rat VAMP2. The sequence is completely conserved in mouse, human, bovine, and Xenopus. |
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| UniProt ID: | P63045 |
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| GenBank ID: | M24105 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Guinea pig, Rabbit
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| Applications: | ICC, IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~18kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicles. Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system. VAMP2 is found in neurons associated with autonomic, sensory and integrative functions, and in non-neuronal Langerhans islets and glomerular cells. VAMP is a component of a 20S complex implicated in vesicle docking and fusion, the formation of which involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of VAMP2, pAb: Lane 1: Molecular Weight Marker, Lane 2: Mouse Brain Tissue Extract (Prod. No.: ADI-LYT-MB100), Lane 3: Rat Brain Tissue Extract (Prod. No.: ADI-LYT-RB100)
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Product Literature References
ELKS/Voltage-Dependent Ca2+ Channel-β Subunit Module Regulates Polarized Ca2+ Influx in Pancreatic β Cells: M. Ohara-Imaizumi, et al.; Cell Rep.
26, 1213 (2019),
Abstract;
Pancreatic acinar cells express vesicle-associated membrane protein 2-and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion: G. Groblewski, et al. ; J. Biol. Chem.
282, 9635 (2007),
Application(s): WB, IP using rat tissue,
Abstract;
Cytoplasmic dynein participates in apically targeted stimulated secretory traffic in primary rabbit lacrimal acinar epithelial cells: S.F. Hamm-Alvarez, et al. ; J. Cell. Sci.
116, 2051 (2003),
Application(s): WB using rabbit samples,
Abstract;
Ca2+-dependent formation of a dynamin-synaptophysin complex: potential role in synaptic vesicle endocytosis: E.B. Ziff, et al. ; J. Biol. Chem.
277, 9010 (2002),
Application(s): IP, WB using rat samples,
Abstract;
Subcellular distribution of 3 functional platelet SNARE proteins: human cellubrevin, SNAP-23, and syntaxin 2: D. Feng, et al.; Blood
99, 4006 (2002),
Application(s): WB using bovine & human samples,
Abstract;
Transduction of MIN6 beta cells with TAT-syntaxin SNARE motif inhibits insulin exocytosis in biphasic insulin release in a distinct mechanism analyzed by evanescent wave microscopy: S. Nagamatsu, et al. ; J. Biol. Chem.
277, 50805 (2002),
Application(s): IP using mouse samples,
Abstract;
Perinuclear localization and insulin responsiveness of GLUT4 requires cytoskeletal integrity in 3T3-L1 adipocytes: M.P. Czech, et al. ; J. Biol. Chem.
275, 38151 (2000),
Application(s): WB using mouse samples,
Abstract;
Human granulocytic ehrlichiosis agent and Ehrlichia chaffeensis reside in different cytoplasmic compartments in HL-60 cells: Y. Rikihisa, et al. ; Infect. Immun.
67, 1368 (1999),
Application(s): ICC using human samples,
Abstract;
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