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SNAP-25 polyclonal antibody

ADI-VAP-SV002-E 100 µg 276.00 USD
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Product Details

Alternative Name:Synaptosomal-associated protein 25
Immunogen:Synthetic peptide corresponding to a portion of mouse SNAP-25. The sequence is completely conserved in human, chicken, and goldfish.
UniProt ID:P60879
GenBank ID:M22012
Source:Purified from rabbit serum.
Species reactivity:Mouse, Rat
Applications:IF, IHC, WB
Recommended Dilutions/Conditions:Western Blot (1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~25kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Synaptic vesicle fusion reactions are mediated by a variety of SNAP proteins, including alpha and beta SNAPs and SNAP-25. Together with synaptotagmin and NSF, alpha-SNAP modulates the interaction of SNAP25, VAMP, and syntaxin in an ATP-dependent manner to form a 20S complex associated with synaptic vesicle docking and fusion. beta-SNAP interacts with synaptotagmin and may be involved in calcium-regulated exocytosis.
Regulatory Status:RUO - Research Use Only

Product Literature References

Cell-autonomous TrkB signaling in presynaptic retinal ganglion cells mediates axon arbor growth and synapse maturation during the establishment of retinotectal synaptic connectivity: S. Marshak, et al.; J. Neurosci. 27, 2444 (2007), Application(s): IHC using xenopus tissue, Abstract;
Neuronal differentiation and synaptogenesis in retinoblastoma: D.A. Johnson, et al.; Cancer Res. 67, 2701 (2007), Application(s): IHC, IF using mouse tissue & tissue culture, Abstract;
The role of zinc binding in the biological activity of botulinum toxin: S. Hao, et al. ; J. Biol. Chem. 276, 27034 (2001), Application(s): WB using mouse & rat samples, Abstract;
Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF: S. Cohen-Cory, et al. ; Nat. Neurosci. 4, 1093 (2001), Application(s): IHC using xenopus samples, Abstract;
The C terminus of SNAP25 is essential for Ca(2+)-dependent binding of synaptotagmin to SNARE complexes: T.F. Martin, et al. ; J. Biol. Chem. 275, 6328 (2000), Application(s): WB using rat samples, Abstract;

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