Product Specification
| Alternative Name: | Synaptosomal-associated protein 25 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to a portion of mouse SNAP-25. The sequence is completely conserved in human, chicken, and goldfish. |
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| UniProt ID: | P60879 |
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| GenBank ID: | M22012 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Mouse, Rat
Xenopus
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| Applications: | IF, IHC, WB
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| Recommended Dilutions/Conditions: | Western Blot (1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~25kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Synaptic vesicle fusion reactions are mediated by a variety of SNAP proteins, including alpha and beta SNAPs and SNAP-25. Together with synaptotagmin and NSF, alpha-SNAP modulates the interaction of SNAP25, VAMP, and syntaxin in an ATP-dependent manner to form a 20S complex associated with synaptic vesicle docking and fusion. beta-SNAP interacts with synaptotagmin and may be involved in calcium-regulated exocytosis. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cell-autonomous TrkB signaling in presynaptic retinal ganglion cells mediates axon arbor growth and synapse maturation during the establishment of retinotectal synaptic connectivity: S. Marshak, et al.; J. Neurosci.
27, 2444 (2007),
Application(s): IHC using xenopus tissue,
Abstract;
Neuronal differentiation and synaptogenesis in retinoblastoma: D.A. Johnson, et al.; Cancer Res.
67, 2701 (2007),
Application(s): IHC, IF using mouse tissue & tissue culture,
Abstract;
The role of zinc binding in the biological activity of botulinum toxin: S. Hao, et al. ; J. Biol. Chem.
276, 27034 (2001),
Application(s): WB using mouse & rat samples,
Abstract;
Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF: S. Cohen-Cory, et al. ; Nat. Neurosci.
4, 1093 (2001),
Application(s): IHC using xenopus samples,
Abstract;
The C terminus of SNAP25 is essential for Ca(2+)-dependent binding of synaptotagmin to SNARE complexes: T.F. Martin, et al. ; J. Biol. Chem.
275, 6328 (2000),
Application(s): WB using rat samples,
Abstract;
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