Product Details
| Alternative Name: | N-Ethylmaleimide-sensitive fusion protein |
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| Clone: | 9G7-3 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | Recombinant bovine NSF. |
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| UniProt ID: | P46459 (human) |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Chicken, Dog, Hamster, Monkey, Porcine, Rabbit, Sheep
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~76kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | N-ethylmaleimide-sensitive factor (NSF) is involved in a variety of membrane fusion events and plays a prominent role in synaptic vesicle fusion. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). Together with synaptotagmin and alpha-SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin in an ATP-dependent manner to form a 20S complex involved in synaptic vesicle docking and fusion. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis: Lane 1: MW marker, Lane 2: HeLa (Heat Shocked), Lane 3: Vero, Lane 4: CHO-K1, Lane 5: ESK-4.
Immunohistochemistry analysis of human testis tissue stained with NSF, mAb (9G7-3) at 20µg/ml.
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Product Literature References
A quantitative study on the expression of synapsin II and N-ethylmaleimide-sensitive fusion protein in schizophrenic patients: H. Nawa, et al. ; Neurosci. Lett.
305, 185 (2001),
Application(s): WB using human samples,
Abstract;
Three novel proteins of the syntaxin/SNAP-25 family: R.H. Scheller, et al. ; J. Biol. Chem.
273, 34171 (1998),
Application(s): WB using monkey samples,
Abstract;
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