VAMP2 monoclonal antibody (SP10)



ADI-VAM-SV014-C	25 µl	216.00 USD

ADI-VAM-SV014-E	100 µl	362.00 USD
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Product Details

Alternative Name:	Synaptobrevin, Vesicle-associated membrane protein 2

Clone:	SP10

Host:	Mouse

Isotype:	IgM

Immunogen:	Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin monoclonal antibodies.

UniProt ID:	P23763 (VAMP1), P63027

GenBank ID:	M36200 (VAMP1), M36205 (VAMP2)

Source:	Purified from ascites.

Species reactivity:	Human, Mouse, Rat Bovine, Hamster, Porcine, Xenopus

Applications:	IHC, WB

Recommended Dilutions/Conditions:	Immunohistochemistry (1:50, human neuroblastoma tissue) Western Blot (1:4,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~18kDa by Western blot.

Purity Detail:	Ammonium sulfate precipitation purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicles. Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system. VAMP2 is found in neurons associated with autonomic, sensory and integrative functions, and in non-neuronal Langerhans islets and glomerular cells. VAMP is a component of a 20S complex implicated in vesicle docking and fusion, the formation of which involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF.

Regulatory Status:	RUO - Research Use Only

VAMP2 monoclonal antibody (SP10) Immunohistochemistry

Immunohistochemistry analysis of Xenopus embryonic brain, stained with VAMP2, mAb (SP10).

Immunohistochemistry analysis of human neuroblastoma tissue immunohistochemically stained using VAMP mAb (SP10) at a dilution of 1:50.

VAMP2 monoclonal antibody (SP10) Western blot

Western blot analysis of mouse brain tissue extract (Prod. No. ADI-LYT-MB100) probed with VAMP2 (Synaptobrevin) mAb.

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Product Literature References

Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles: B.P. Jena, et al. ; PNAS 99, 4720 (2002), Application(s): WB using rat samples, Abstract;

The 26-mer peptide released from SNAP-25 cleavage by botulinum neurotoxin E inhibits vesicle docking: M. Montal, et al. ; FEBS Lett. 435, 84 (1998), Application(s): WB using rat samples, Abstract;

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