Product Details
| Alternative Name: | SYP |
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| Clone: | SP15 |
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| Host: | Mouse |
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| Isotype: | IgM |
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| Immunogen: | Synaptic vesicle-containing fractions immunoprecipitated from human brain homogenates using anti-human Synaptophysin mAb antibodies. |
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| UniProt ID: | P08247 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine
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| Applications: | IHC, WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (1:50, human neuroblastoma tissue)
Western Blot (1:2,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~38kDa by Western blot. |
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| Purity Detail: | Ammonium sulfate precipitation purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Synaptophysin is an integral membrane protein involved in neurotransmitter exocytosis. This protein consists of four transmembrane domains, with its N- and C-terminus facing the cytoplasm. Studies have shown Synaptophysin to be a major cholesterol-binding protein in brain synaptic vesicles. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Synaptophysin, mAb (SP15) (Prod. No. ADI-VAM-SV011): Lane 1: MW marker; Lane 2: Mouse brain extract (Prod. No. ADI-LYT-MB100); Lane 3: Rat brain extract (Prod. No. ADI-LYT-RB100)
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Product Literature References
The development of mastication in rodents: From neurons to behaviors: J.E. Turman Jr., et al.; Arch. Oral Biol.
52, 313 (2007),
Application(s): IHC using mouse tissue,
Abstract;
Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex: P. Davies, et al. ; Brain Res.
609, 20 (1993),
Application(s): WB using human samples,
Abstract;
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