Product Details
Alternative Name: | GOSR1, Golgi SNAP receptor complex member 1 |
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Clone: | HFD9 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Rat liver Golgi-enriched membranes. |
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UniProt ID: | Q62931 |
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Source: | Purified from ascites. |
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Species reactivity: | Mouse, Rat Bovine, Chicken, Dog, Hamster, Porcine, Rabbit, Sheep
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Applications: | WB Electron microscopy
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~28kDa by Western blot. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Golgi SNARE of 28 kDa (GS28), also known as p28 or GOS28, is a 28 kDa integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport. The amino-terminal of GS28 is exposed to the cytosol and is anchored to the cis Golgi via a 20 amino acid carboxyl-terminal hydrophobic tail. GS28 co-immunoprecipitates complexes consisting of Syntaxin 5, Rbet1, Membrin, Rsec22, and Rsly1, and is therefore implicated in ER to-Golgi or intra-Golgi vesicle transport. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of pig kidney ESK-4 cell lysate, probed with GS28 mAb (HFD9).
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Product Literature References
Data supporting ER stress response in NG108-15 cells involves upregulation of syntaxin 5 expression and reduced amyloid β peptide secretion: K. Suga, et al.; Data Brief
5, 782 (2015),
Application(s): Western blot,
Abstract;
ER stress response in NG108-15 cells involves upregulation of syntaxin 5 expression and reduced amyloid β peptide secretion: K. Suga, et al.; Exp. Cell Res.
332, 11 (2015),
Application(s): Western Blotting,
Abstract;
Sterols block binding of COPII proteins to SCAP, thereby controlling SCAP sorting in ER: D. Yabe, et al. ; PNAS
99, 11694 (2002),
Application(s): EM using human samples,
Abstract;
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