Product Details
| Clone: | SAP7F407 |
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| Host: | Mouse |
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| Isotype: | IgG2aκ |
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| Immunogen: | Recombinant rat Bassoon. |
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| UniProt ID: | O88778 |
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| Gene/Protein Identifier: | 29999 (UniGene) |
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| Source: | Purified from ascites. |
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| Species reactivity: | Mouse, Rat
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| Applications: | ICC, IF, IHC, IP, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:400)
Immunoprecipitation (2.5µg)
Western Blot (1:500 - 1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~400kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Bassoon is a 420 kDa protein that localizes at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release. |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of bassoon localization in the rat brain cerebellar molecular layer using Bassoon, mAb (SAP7F407).
Western blot analysis of Bassoon: Lane 1: MW marker, Lane 2: Rat Brain Tissue Lysate.
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Product Literature References
Immunohistochemical distribution of proteins involved in glutamate release in subepithelial sensory nerve endings of rat epiglottis: Y. Yamamoto, et al.; Histochem. Cell Biol.
157, 51 (2022),
Abstract;
Immunohistochemical localization of vesicular nucleotide transporter in small intensely fluorescent (SIF) cells of the rat superior cervical ganglion: T. Yokoyama, et al.; Tissue Cell
79, 101924 (2022),
Abstract;
KIF2C regulates synaptic plasticity and cognition in mice through dynamic microtubule depolymerization: R. Zheng, et al.; Elife
11, e72483 (2022),
Abstract;
A stable proportion of Purkinje cell inputs from parallel fibers are silent during cerebellar maturation: S. Ho, et al.; PNAS
118, e2024890118 (2021),
Abstract;
An engineered channelrhodopsin optimized for axon terminal activation and circuit mapping: S. Hamada, et al.; Commun. Biol.
4, 461 (2021),
Abstract;
Full Text
Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale Study: J. Jablonski, et al.; Cells
10, 3004 (2021),
Abstract;
Loss of miR-183/96 alters synaptic strength via pre- and postsynaptic mechanisms at a central synapse: C. Kros, et al.; J. Neurosci.
41, 6796 (2021),
Abstract;
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission: N. Lipstein, et al.; Neuron
109, 3980 (2021),
Abstract;
SorCS2 is required for social memory and trafficking of the NMDA receptor: J. Yang, et al.; Mol. Psychiatry
26, 927 (2021),
Application(s): Immunofluorescence using mouse neurons,
Abstract;
The lytic siphophage vB_StyS-LmqsSP1 reduces Typhimurium isolates on chicken skin: G. Shakeri, et al.; Appl. Environ. Microbiol.
87, e0142421 (2021),
Abstract;
Blockade of Glial Connexin 43 Hemichannels Reduces Food Intake: F. Guillebaud, et al.; Cells
9, 2387 (2020),
Abstract;
Full Text
Protocol for quantitative analysis of synaptic vesicle clustering in axons of cultured neurons: J. W. Um, et al.; STAR Protoc.
1, 100095 (2020),
Application(s): Immunofluorescence using mouse neurons,
Abstract;
Full Text
Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones: S. Siddig, et al.; Sci. Adv.
6, eaay7193 (2020),
Application(s): Immunofluorescence using mouse brain tissue sections,
Abstract;
Full Text
Synaptotagmin-1 is the Ca 2+ sensor for fast striatal dopamine release: A. Banerjee, et al.; Elife
9, e58359 (2020),
Abstract;
Full Text
Ultrastructural correlates of presynaptic functional heterogeneity in hippocampal synapses: L. Maus, et al.; Cell Rep.
30, 3632 (2020),
Application(s): Immunofluorescence using mouse hippocampal tissue sections,
Abstract;
Full Text
Vesicular nucleotide transporter-immunoreactive type I cells associated with P2X3-immunoreactive nerve endings in the rat carotid body: T. Yokoyama, et al.; J. Comp. Neurol.
528, 1486 (2020),
Abstract;
CNS synapses are stabilized trans-synaptically by laminins and laminin-interacting proteins: D.D. Hunter, et al.; J. Comp. Neurol.
527, 67 (2019),
Abstract;
ELKS/Voltage-Dependent Ca2+ Channel-β Subunit Module Regulates Polarized Ca2+ Influx in Pancreatic β Cells: M. Ohara-Imaizumi, et al.; Cell Rep.
26, 1213 (2019),
Abstract;
Synaptic FUS Localization During Motoneuron Development and Its Accumulation in Human ALS Synapses: D. Deshpande, et al.; Front. Cell. Neurosci.
13, 256 (2019),
Abstract;
Full Text
The Na+/H+-Exchanger NHE1 Regulates Extra- and Intracellular pH and Nimodipine-sensitive [Ca2+]i in the Suprachiasmatic Nucleus: P.C. Cheng, et al.; Sci. Rep.
9, 6430 (2019),
Abstract;
Full Text
Ablation of the presynaptic organizer Bassoon in excitatory neurons retards dentate gyrus maturation and enhances learning performance: A. Annamneedi, et al.; Brain Struct. Funct.
223, 3423 (2018),
Abstract;
Full Text
Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice: H. Nishimune, et al.; Sci. Rep.
6, 27935 (2018),
Abstract;
Full Text
Localization of nectin-2α at the boundary between the adjacent somata of the clustered cholinergic neurons and its regulatory role in the subcellular localization of the voltage-gated A-type K+ channel Kv4.2 in the medial habenula: H. Shiotani, et al.; J. Comp. Neurol.
526, 1527 (2018),
Abstract;
Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins: T. Cijsouw, et al.; Proteomes
28, E48 (2018),
Abstract;
Full Text
Structural insights into modulation and selectivity of transsynaptic neurexin-LRRTM interaction: A. Yamagata, et al.; Nat. Commun.
9, 3964 (2018),
Abstract;
Full Text
Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Müller Glial Reactivity: S. Koh, et al.; J. Neurosci.
38, 2923 (2018),
Abstract;
Full Text
The presynaptic scaffolding protein Piccolo organizes the readily releasable pool at the calyx of Held: D. Parthier, et al.; J. Physiol.
596, 1485 (2018),
Abstract;
α-Actinin Anchors PSD-95 at Postsynaptic Sites: L. Matt, et al.; Neuron
97, 1094 (2018),
Abstract;
A competitive inhibitory circuit for selection of active and passive fear responses: J.P. Fadok, et al.; Nature
542, 96 (2017),
Abstract;
A single dose of docosahexaenoic acid increases the functional recovery promoted by rehabilitation following cervical spinal cord injury in the rat: Z.H. Liu, et al.; J. Neurotrauma
34, 1766 (2017),
Abstract;
Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors: C. Puller, et al.; PLoS One
12, e0173455 (2017),
Abstract;
Full Text
Monoacylglycerol lipase inhibitor JZL184 prevents HIV-1 gp120-induced synapse loss by altering endocannabinoid signaling: X. Zhang, et al.; Neuropharmacology
128, 269 (2017),
Abstract;
Multiple roles of afadin in the ultrastructural morphogenesis of mouse hippocampal mossy fiber synapses: K. Sai, et al.; J. Comp. Neurol.
525, 2719 (2017),
Abstract;
Na⁺/Ca²⁺ Exchanger 2 (NCX2) in the Circadian Clock of the Rat Suprachiasmatic Nucleus: Colocalization with Neuropeptides and Daily Profiles of Gene Expression and Protein Levels: P.C. Cheng, et al.; Chin. J. Physiol.
60, 215 (2017),
Abstract;
N‐terminal SAP97 isoforms differentially regulate synaptic structure and postsynaptic surface pools of AMPA receptors: L. Goodman, et al.; Hippocampus
27, 668 (2017),
Abstract;
Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice: C. Vicidomini, et al.; Mol. Psychiatry
22, 689 (2017),
Abstract;
Full Text
Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues: Y. Wang, et al.; Nano Lett.
17, 6131 (2017),
Abstract;
Full Text
RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the mammalian brain: P. Valnegri, et al.; Nat. Commun.
8, 1271 (2017),
Abstract;
Full Text
CASK stabilizes neurexin and links it to liprin-α in a neuronal activity-dependent manner: L.E. Laconte, et al.; Cell. Mol. Life Sci.
73, 3599 (2016),
Abstract;
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking: S.S. Wang, et al.; Neuron
91, 777 (2016),
Application(s): Immunostaining, Immunoblotting,
Abstract;
Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury: S. Kawabata, et al. ; Stem Cell Reports
6, 1 (2016),
Application(s): Immunohistochemistry,
Abstract;
Impairment of Vision in a Mouse Model of Usher Syndrome Type III: G. Tian, et al.; Invest. Ophthalmol. Vis. Sci.
57, 866 (2016),
Application(s): Immunofluorescence staining,
Abstract;
Full Text
Long time remodeling during retinal degeneration evaluated by optical coherence tomography, immunocytochemistry and fundus autofluorescence: I. Pinilla, et al.; Exp. Eye Res.
150, 122 (2016),
Abstract;
Periodic F-actin structures shape the neck of dendritic spines: J. Bär, et al.; Sci. Rep.
6, 37136 (2016),
Abstract;
Full Text
RASGRF2 controls nuclear migration in postnatal retinal cone photoreceptors: D. Jimeno, et al.; J. Cell Sci.
129, 729 (2016),
Abstract;
Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus: C. Heise, et al.; Front. Cell. Neurosci.
10, 106 (2016),
Abstract;
Full Text
The bacterial toxin CNF1 as a tool to induce retinal degeneration reminiscent of retinitis pigmentosa: V. Guadagni, et al.; Sci. Rep.
6, 35919 (2016),
Abstract;
Full Text
Three-dimensional architectures of P2X2-/P2X3-immunoreactive afferent nerve terminals in the rat carotid body as revealed by confocal laser scanning microscopy: T. Yokoyama, et al.; Histochem. Cell Biol.
146, 479 (2016),
Abstract;
Visualizing K48 Ubiquitination during Presynaptic Formation By Ubiquitination-Induced Fluorescence Complementation (UiFC): M.J. Pinto, et al.; Front. Mol. Neurosci.
9, 43 (2016),
Abstract;
Full Text
Dopaminergic control of autophagic-lysosomal function implicates Lmx1b in Parkinson's disease: A. Laguna, et al.; Nat. Neurosci.
18, 826 (2015),
Application(s): Immunohistochemistry,
Abstract;
GSK3β Inhibition Promotes Synaptogenesis in Drosophila and Mammalian Neurons: G. Cuesto, et al.; PLoS One
10, e0118475 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
Mechanisms of splicing-dependent trans-synaptic adhesion by PTPδ-IL1RAPL1/IL-1RAcP for synaptic differentiation: A. Yamagata, et al.; Nat. Commun.
6, 6926 (2015),
Application(s): Immunostaining,
Abstract;
Full Text
The phospholipid-binding protein SESTD1 negatively regulates dendritic spine density by interfering with Rac1-Trio8 signaling pathway: C. Lee, et al.; Sci. Rep.
5, 13250 (2015),
Application(s): Immunofluorescence,
Abstract;
TIMP-1 affects the spatial distribution of dendritic processes of second-order neurons in a rat model of Retinitis Pigmentosa: J. Shin, et al. ; Exp. Eye Res.
140, 41 (2015),
Application(s): Immunohistochemistry,
Abstract;
Acute mechanisms underlying antibody effects in anti–N‐methyl‐D‐aspartate receptor encephalitis: E.H. Moscato, et al.; Ann. Neurol.
76, 108 (2014),
Application(s): Immunocytochemistry using rat hippocampal neurons,
Abstract;
Full Text
ArfGAP3 is a component of the photoreceptor synaptic ribbon complex and forms an NAD(H)-regulated, redox-sensitive complex with RIBEYE that is important for endocytosis: M. Dembla, et al.; J. Neurosci.
34, 5245 (2014),
Application(s): Immunohistochemistry using murine retina sections,
Abstract;
Full Text
LKB1 and AMPK regulate synaptic remodeling in old age: M.A. Samuel, et al.; Nat. Neurosci.
17, 1190 (2014),
Application(s): Immunohistochemistry and Western blot using murine retina tissue samples,
Abstract;
Full Text
Co-regulation of intragenic microRNA miR-153 and its host gene Ia-2β: identification of miR-153 target genes with functions related to IA-2β in pancreas and brain: W. Mandemakers, et al.; Diabetologia
56, 1547 (2013),
Application(s): WB analysis of endogenous bassoon in SNAP25, SNCA, parkin and beta actin from SH-SY5Y cells,
Abstract;
Full Text
Human immunodeficiency virus-1 protein Tat induces excitotoxic loss of presynaptic terminals in hippocampal cultures: A.H. Shin & S.A. Thayer; Mol. Cell. Neurosci.
54, 22 (2013),
Application(s): IHC on mouse hippocampal slice cultures,
Abstract;
Full Text
N-Cadherin Relocalizes from the Periphery to the Center of the Synapse after Transient Synaptic Stimulation in Hippocampal Neurons: P.T. Yam, et al.; PLoS One
8, e79679 (2013),
Application(s): Immunostaining Bassoon staining at synapses in paraformaldehyde fixed neurons from Sprague Dawley rats,
Abstract;
Full Text
Active Zone Protein Bassoon Co-Localizes With Presynaptic Calcium Channel, Modifies Channel Function, and Recovers From Aging Related Loss by Exercise: H. Nishimune, et al.; PLoS One
7, e38029 (2012),
Abstract;
Full Text
Protein composition of immunoprecipitated synaptic ribbons: A. Kantardzhieva, et al. ; J. Proteome Res.
11, 1163 (2012),
Application(s): Immunolabeling of mouse retinas,
Abstract;
Full Text
Calcium Channels Link the Muscle-Derived Synapse Organizer Laminin β2 to Bassoon and CAST/Erc2 to Organize Presynaptic Active Zones: J. Chen, et al.; J. Neurosci.
21, 512 (2011),
Application(s): Visualized by fluorescent immunohistochemistry using an anti-Bassoon antibody,
Abstract;
Full Text
γ-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina: J. Sanes, et al. ; Development
135, 4141 (2008),
Application(s): IHC using mouse tissue,
Abstract;
Mechanisms of prolonged presynaptic Ca(2+) signaling and glutamate release induced by TRPV1 activation in rat sensory neurons: Y. Usachev, et al. ; J. Neurosci.
28, 5295 (2008),
Application(s): ICC, IF using rat tissue culture,
Abstract;
Polarized Targeting of Neurexins to Synapses Is Regulated by their C-Terminal Sequences: M. Missler, et al. ; J. Neurosci.
28, 12969 (2008),
Application(s): IHC using mouse tissue culture,
Abstract;
Retinal cells integrate into the outer nuclear layer and differentiate into mature photoreceptors after subretinal transplantation into adult mice: M. Ader, et al. ; Exp. Eye Res.
86, 691 (2008),
Application(s): IHC, IF using mouse tissue,
Abstract;
Extensive neuronal differentiation of human neural stem cell grafts in adult rat spinal cord: V. Koliatsos, et al. ; PLoS Med.
4, 318 (2007),
Application(s): IHC using rat tissue,
Abstract;
Neuronal differentiation and synaptogenesis in retinoblastoma: D.A. Johnson, et al.; Cancer Res.
67, 2701 (2007),
Application(s): IHC, IF using mouse tissue & tissue culture,
Abstract;
Ultrastructural localization of active zone and synaptic vesicle proteins in a preassembled multi-vesicle transport aggregate.: J.H.Tao-Cheng, et al.; Neuroscience
150, 575 (2007),
Application(s): Immunogold labeling of Bassoon in dissociated hippocampal neuronal cultures,
Abstract;
Full Text
Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory: R.L. Huganir, et al. ; Cell
112, 631 (2003),
Application(s): ICC using mouse samples,
Abstract;
Rapid and differential regulation of AMPA and kainate receptors at hippocampal mossy fibre synapses by PICK1 and GRIP: J.M. Henley, et al. ; Neuron
37, 625 (2003),
Application(s): WB using rat samples,
Abstract;
CAST: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1: Y. Takai, et al. ; J. Cell Biol.
158, 577 (2002),
Application(s): IP, WB, IHC using rat & mouse samples,
Abstract;
The presynaptic particle web: ultrastructure, composition, dissolution, and reconstitution: D.R. Colman, et al. ; Neuron
32, 63 (2001),
Application(s): WB using rat samples,
Abstract;
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