Product Details
| Alternative Name: | HSC70/HSP90-organizing protein, STIP1, STI1, Stress-induced phosphoprotein 1, STUB1, p60, CHIP |
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| Recommended Dilutions/Conditions: | Western Blot (50ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| MW: | ~63kDa |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P31948 |
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| Formulation: | Liquid. In Dulbecco's PBS. |
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| Purity: | ≥90% (SDS-PAGE; Western blot) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Applications: | WB
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| Application Notes: | Western blot control. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | Hsp70-Hsp90 Organizing Protein (HOP, p60) is an ~60kDa protein that is a critical intermediate component for the efficient maturation of steroid receptor complexes, serving to recruit Hsp90 to Hsp70-containing complexes. Unactivated steroid hormone receptors are found in hetero-oligomeric complexes that are thought to stabilize a partially folded receptor polypeptide prior to hormone - dependent activation. Hsp70 and Hsp90 are both known to associate with steroid hormone receptors. Numerous studies have shown that Hsp70 functions in an ATPdependent manner through transient interactions to mediate folding or unfolding of polypeptide chains. Hsp90 is thought to perhaps also function in some capacity related to folding or protein-protein interactions. HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. Hsp70 binding has been localized to TPR1, and sp90 binding has been localized to TPR2a. Importantly, the highly conserved EEVD sequence that terminates many Hsp70 family members, and the similar MEEVD sequence that terminates Hsp90, are important recognition sites for the TPR domains. The co-crystal structures for TPR1 plus a GPTIEEVD octapeptide (Hsp70 sequence) and TPR2a plus the MEEVD pentapeptide (Hsp90) have been solved. HOP is closely related to a human 63 kDa protein that is sensitive to simian virus SV40 transformation and is related to the yeast heat shock-responsive STI1 gene product. |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 1µg, Lane 3: 2µg, Lane 4: 5µg HOP Recombinant Protein.
Western Blot analysis: Lane 1: MWM, Lane 2: HOP Recombinant Protein, Lane 3: HeLa cell lysate.
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Product Literature References
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol.
97, 518 (2015),
Application(s): Western Blotting,
Abstract;
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