Estrogen receptor α monoclonal antibody (C-542)



ADI-SRA-1010-D	50 µg	243.00 USD

ADI-SRA-1010-F	200 µg	501.00 USD
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Product Details

Alternative Name:	ESR

Clone:	C-542

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to the sequence near the C-terminus of human Estrogen Receptor α.

UniProt ID:	P03372

GenBank ID:	M12674

Source:	Purified from ascites.

Species reactivity:	Human, Mouse, Rat Hamster

Applications:	ICC, IHC, IP, WB

Recommended Dilutions/Conditions:	Western Blot (1:500, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of 66.3kDa by Western blot.

Purity Detail:	Protein G affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues.

Regulatory Status:	RUO - Research Use Only

Estrogen receptor alpha monoclonal antibody (C-542) Schematic structure

Human Estrogen Alpha Receptor domains.

Estrogen receptor alpha monoclonal antibody (C-542) Western blot

Western blot analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).

Estrogen receptor alpha monoclonal antibody (C-542) IHC

Immunohistochemistry analysis of human Breast tumor tissue stained with Estrogen receptor α monoclonal antibody (C-542), (Prod. No. ADI-SRA-1010), at 20µg/ml.

Estrogen receptor alpha monoclonal antibody (C-542) ICC

Immunocytochemistry staining of HepG2 cells (Panel A) and MCF-7 (Panel B) with Estrogen receptor α monoclonal antibody (C-542) (Prod. No. ADI-SRA-1010) at 10µg/ml.

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Product Literature References

Chronic exposure to benzyl butyl phthalate (BBP) alters social interaction and fear conditioning in male adult rats: alterations in amygdalar MeCP2, ERK1/2 and ERα: A. Betz, et al.; Neuro. Endocrinol. Lett. 34, 347 (2013), Abstract;

Reassessment of estrogen receptor expression in human breast cancer cell lines: C.H. Ford, et al.; Anticancer Res. 31, 521 (2011), Application(s): Flow cytometry and immunofluorescence, Abstract;

Membrane estrogen receptor-α levels in MCF-7 breast cancer cells predict cAMP and proliferation responses: D. Zivadinovic, et al.; Breast Cancer Res. 7, R101 (2005), Application(s): Cell immunoseparation, Abstract;

Regulation of the membrane estrogen receptor-alpha: role of cell density, serum, cell passage number, and estradiol: C.S. Watson, et al. ; FASEB J. 16, 1917 (2002), Application(s): ICC using rat samples, Abstract;

The novel isoform of the estrogen receptor-alpha cDNA (ERalpha isoform S cDNA) in the human testis: K. Hoshi, et al. ; J. Steroid Biochem. Mol. Biol. 80, 299 (2002), Application(s): WB using human samples, Abstract;

Functional interactions between the estrogen receptor and DRIP205, a subunit of the heteromeric DRIP coactivator complex: L.P. Freedman, et al. ; J. Biol. Chem. 275, 20928 (2000), Application(s): IP, WB using human samples, Abstract;

Estrogen receptor-dependent regulation of sensory neuron survival in developing dorsal root ganglion: D. Lindholm, et al. ; PNAS 96, 10905 (1999), Application(s): ICC using rat samples, Abstract;

Characterization of Brx, a novel Dbl family member that modulates estrogen receptor action: J. Segars, et al. ; Oncogene 16, 2513 (1998), Application(s): IP, IHC using human samples, Abstract;