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Estrogen receptor monoclonal antibody (h-151)

ADI-SRA-1000-D 50 µg 225.00 USD
ADI-SRA-1000-F 200 µg 501.00 USD
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Product Details

Alternative Name:ESR
Immunogen:Synthetic peptide corresponding to the sequence of human estrogen receptor, conjugated to KLH.
UniProt ID:P03372
GenBank ID:M12674
Source:Purified from mouse ascites.
Species reactivity:Human, Rat
Applications:ICC, IP, WB
Recommended Dilutions/Conditions:Immunoprecipitation (15µg/ml)
Western Blot (1:250)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~67kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues.
Regulatory Status:RUO - Research Use Only
Estrogen receptor monoclonal antibody (h-151) Schematic structure
Human Estrogen Receptor domains.
Estrogen receptor monoclonal antibody (h-151) Western blot
Western blot analysis of ESR1: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa)
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Estrogen receptor monoclonal antibody (h-151) Schematic structure Estrogen receptor monoclonal antibody (h-151) Western blot

Product Literature References

Reassessment of estrogen receptor expression in human breast cancer cell lines: C.H. Ford, et al.; Anticancer Res. 31, 521 (2011), Application(s): Flow cytometry and immunofluorescence, Abstract;
Serum-dependent effects of tamoxifen and cannabinoids upon C6 glioma cell viability: C.J. Fowler, et al. ; Biochem. Pharmacol. 60, 1807 (2000), Application(s): WB using rat samples, Abstract;
Rapid actions of estrogens in GH3/B6 pituitary tumor cells via a plasma membrane version of estrogen receptor-alpha: B. Gametchu, et al. ; Steroids 64, 13 (1999), Application(s): ICC using human & rat samples, Abstract;
An N-terminal inhibitory function, IF, suppresses transcription by the A-isoform but not the B-isoform of human progesterone receptors: K.B. Horwitz, et al. ; J. Biol. Chem. 273, 5455 (1998), Application(s): WB using human samples, Abstract;
AIB1, a steroid receptor coactivator amplified in breast cancer and ovarian cancer: P.S. Meltzer, et al. ; Science 277, 965 (1997), Application(s): WB using human samples, Abstract;

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