Estrogen receptor monoclonal antibody (h-151)



ADI-SRA-1000-D	50 µg	225.00 USD

ADI-SRA-1000-F	200 µg	501.00 USD
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Product Details

Alternative Name:	ESR

Clone:	h-151

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to the sequence of human estrogen receptor, conjugated to KLH.

UniProt ID:	P03372

GenBank ID:	M12674

Source:	Purified from mouse ascites.

Species reactivity:	Human, Rat

Applications:	ICC, IP, WB

Recommended Dilutions/Conditions:	Immunoprecipitation (15µg/ml) Western Blot (1:250) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~67kDa by Western blot.

Purity Detail:	Protein G affinity purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues.

Regulatory Status:	RUO - Research Use Only

Estrogen receptor monoclonal antibody (h-151) Schematic structure

Human Estrogen Receptor domains.

Estrogen receptor monoclonal antibody (h-151) Western blot

Western blot analysis of ESR1: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa)

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Product Literature References

Reassessment of estrogen receptor expression in human breast cancer cell lines: C.H. Ford, et al.; Anticancer Res. 31, 521 (2011), Application(s): Flow cytometry and immunofluorescence, Abstract;

Serum-dependent effects of tamoxifen and cannabinoids upon C6 glioma cell viability: C.J. Fowler, et al. ; Biochem. Pharmacol. 60, 1807 (2000), Application(s): WB using rat samples, Abstract;

Rapid actions of estrogens in GH3/B6 pituitary tumor cells via a plasma membrane version of estrogen receptor-alpha: B. Gametchu, et al. ; Steroids 64, 13 (1999), Application(s): ICC using human & rat samples, Abstract;

An N-terminal inhibitory function, IF, suppresses transcription by the A-isoform but not the B-isoform of human progesterone receptors: K.B. Horwitz, et al. ; J. Biol. Chem. 273, 5455 (1998), Application(s): WB using human samples, Abstract;

AIB1, a steroid receptor coactivator amplified in breast cancer and ovarian cancer: P.S. Meltzer, et al. ; Science 277, 965 (1997), Application(s): WB using human samples, Abstract;

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