Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~60kDa by Western blot.
Purity Detail:
Protein A affinity purified.
Formulation:
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Scientific Background:
GroEL and GroES are E. coli chaperonins, which are homologs of mitochondrial chaperonins Hsp60 and Hsp10. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding with an ATP-dependent mechanism.
Regulatory Status:
RUO - Research Use Only
Western Blot Analysis of GroEL: Lane 1: MW Marker, Lane 2: GroEL (E. coli), (recombinant) (Prod. No. ADI-SPP-610), Lane 3: HSP60 (human), (recombinant) (Prod. No. ADI-NSP-540), Lane 4: HeLa (Heat Shocked), Cell Lysate (Prod. No. ADI-LYC-HL101), Lane 5: E. coli Cell Lysate all probed with GroEL (E. coli), pAb (Prod. No. ADI-SPS-875).
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Product Literature References
Protein AMPylation by an Evolutionarily Conserved Pseudokinase: A. Sreelatha, et al.; Cell 175, 809 (2018), Abstract;
The Bradyrhizobium japonicum ferrous iron transporter FeoAB is required for ferric iron utilization in free-living aerobic cells and for symbiosis: S. Sankari, et al.; J. Biol. Chem. 291, 15653 (2016), Application(s): Western-blot, Abstract; Full Text
Substrates control multimerization and activation of the multi-domain ATPase motor of type VII secretion: O.S. Rosenberg, et al.; Cell 161, 501 (2015), Application(s): Western-blot, Abstract; Full Text
