Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Thioredoxin (E. coli), (recombinant) (His-tag)

ADI-SPP-892-200 200 µg 277.00 USD
ADI-SPP-892-1 1 mg 735.00 USD
Do you need bulk/larger quantities?

Product Details

Alternative Name:Trx
Recommended Dilutions/Conditions:Activity Assay (>300U/mg)
Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Source:Produced in E. coli. E. coli thioredoxin is fused at the N-terminus to a His-tag.
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 100mM sodium chloride and 1mM EDTA.
Purity:≥95% (SDS-PAGE; Western blot)
Purity Detail:Purified by multi-step chromatography.
Activity assay
Application Notes:Western blot control.
Shipping:Dry Ice
Long Term Storage:-80°C
Scientific Background:Thioredoxin (Trx) is a redox protein of approximately 12 kDa. Its primary domain is conserved across a number of Trx family members and contains a conserved catalytic site Cys-Gly-Pro-Cys. It is ubiquitous and found in many organisms from bacteria to mammals. Trx has been shown to function in cell proliferation, redox signaling and inhibition of apoptosis.
Trx is reduced by the NADPH-dependent flavoenzyme thioredoxin reductase, and in turn reduces other proteins such as protein disulfide isomerase (PDI) by cysteine thiol-disulfide exchange. Trx has been reported to selectively activate the DNA binding of a number of transcription factors such as p53, NFkB, AP1 and glucocorticoid receptor, resulting in alteration of gene transcription. The reduced Trx can supply reducing equivalents for ribonucleotide reductase, the essential enzyme for DNA synthesis, and thioredoxin peroxidase. As a cofactor for the peroxiredoxins, Trx plays a direct role in reducing oxidative species such as H2O2.
Regulatory Status:RUO - Research Use Only
Thioredoxin (E. coli), (recombinant) (His-tag) SDS-PAGE
SDS-PAGE analysis: Lane 1: MWM, Lane 2: 1µg, Lane 3: 2µg, Lane 4: 5µg Thioredoxin.
Thioredoxin (E. coli), (recombinant) (His-tag) Western blot
Western Blot analysis: Lane 1: MWM, Lane 2: Thioredoxin, probed with thioredoxin mAb.
Thioredoxin (E. coli), (recombinant) (His-tag) image
Variable amounts of thioredoxin incubated with Insulin in the presence of DTT. Activity is measured as increase in absorbance over time.
Please mouse over
Thioredoxin (E. coli), (recombinant) (His-tag) SDS-PAGE Thioredoxin (E. coli), (recombinant) (His-tag) Western blot Thioredoxin (E. coli), (recombinant) (His-tag) image

Product Literature References

Chemoproteomics-Enabled Covalent Ligand Screening Reveals a Thioredoxin-Caspase 3 Interaction Disruptor That Impairs Breast Cancer Pathogenicity: K.E. Anderson, et al.; ACS Chem. Biol. 12, 2522 (2017), Abstract; Full Text

General Literature References

Thioredoxin superfamily and thioredoxin-inducing agents: K. Hirota, et al.; Ann. N. Y. Acad. Sci. 957, 189 (2002), Abstract;
Properties and biological activities of thioredoxins: G. Powis & W.R. Montfort; Annu. Rev. Biophys. Biomol. Struct. 30, 421 (2001), Abstract;

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
Thioredoxin Recombinant protein
By catalog section:


Technical Service
Customer Service

Related Literature

Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Download as PDF

All new literature pieces