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Grp75/Mortalin (human), (recombinant)

ADI-SPP-828-D 50 µg 270.00 USD
ADI-SPP-828-F 200 µg 616.00 USD
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Product Details

Alternative Name:MtHsp70, HspA9, Heat shock 70 kDa protein 9
Recommended Dilutions/Conditions:Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Source:Produced in E. coli.
UniProt ID:P38646
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 3% glycerol and 150mM sodium chloride.
Purity:≥80% (SDS-PAGE; Western blot)
Purity Detail:Purified by multi-step chromatography.
Application Notes:Western blot control.
Shipping:Dry Ice
Long Term Storage:-80°C
Scientific Background:Cells from various organisms increase expression of a class of proteins referred to as heat shock or stress proteins in response to adverse changes in their environment. The members of one class of stress proteins, the Hsp70 family, all bind ATP in vitro, but exist within different intracellular compartments. Members include constitutive form Hsc70 within the cytosol/nucleus; inducible form Hsp70 within the cytosol/nucleus/nucleolus; the constitutive glucose-regulated 78 kDa (or BiP) protein within the lumen of the endoplasmic reticulum; and the constitutive glucose-regulated 75 kDa (Grp75) protein within the mitochondrial matrix. Members of the Hsp70 family appear to function as “molecular chaperones, assisting in the folding of other proteins in various intracellular compartments. Grp75 resides in the mitochondrial matrix where it collaborates with Hsp60 in the re-folding of proteins translocated into this organelle. Like itsE. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone.
Regulatory Status:RUO - Research Use Only
Grp75/Mortalin (human), (recombinant) SDS-PAGE
SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 1µg, Lane 3: 2µg, Lane 4: 5µg of Grp75 (Mortalin) Recombinant Human Protein.
Grp75/Mortalin (human), (recombinant) Western blot
Western Blot analysis: Lane 1: MWM, Lane 2: Grp75 (Mortalin) Recombinant Protein, Lane 3: HeLa Cell Lysate (Heat Shocked) probed with Grp75, pAb.
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Grp75/Mortalin (human), (recombinant) SDS-PAGE Grp75/Mortalin (human), (recombinant) Western blot

Product Literature References

Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus: I. Hassouna, et al.; Mol. Psychiatry 21, 1752 (2016), Application(s): Immunoblot analysis, Abstract;

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