| Alternative Name: | EIF2AK3, Pek |
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| MW: | ~69kDa |
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| Source: | Produced in E. coli. Recombinant human PERK (aa 536-1116). |
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| UniProt ID: | Q9NZJ5 |
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| Gene/Protein Identifier: | NP_004827 (RefSeq) |
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| Formulation: | Liquid. In 50mM TRIS, pH 7.5, containing 100mM sodium chloride, 1mM dithiothreitol, and 20% glycerol. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Applications: | WB
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| Application Notes: | Western blot control. |
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| Shipping: | Shipped on Dry Ice |
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| Short Term Storage: | -80°C |
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| Long Term Storage: | -80°C |
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| Handling: | After reconstitution, prepare aliquots and store at -80°C Avoid freeze/thaw cycles. |
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| Scientific Background: | The endoplasmic reticulum (ER)-resident protein kinase PERK (also known as Pek, or Eukaryotic initiation factor 2 α-subunit kinase 3 (EIF2AK3)) attenuates protein synthesis in response to ER stress through the phosphorylation of translation initiation factor eIF2α on serine 51. A Type I membrane protein located in the ER, PERK is a critical regulator of the unfolded protein response (UPR). Defects in PERK are the cause of Wolcott-Rallison syndrome (WRS). |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE Analysis: Lane 1: MW marker; Lane 2: 1.0 μg of purified PERK protein (Prod. No. ADI-SPP-768).
Western Blot analysis of PERK protein (Prod. No. ADI-SPP-768) probed with anti-PERK antibody. Lane 1: MW marker, Lane2: 50 ng, Lane 3: 100 ng.
Western Blot analysis of PERK protein (Prod. No. ADI-SPP-768) probed with anti-6x His tag antibody. Lane 1: MW marker, Lane2: 50 ng, Lane 3: 100 ng.
