Product Specification
| Alternative Name: | Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8, HSPA8 |
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| MW: | 73kDa (observed) |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P19120 |
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| Formulation: | Liquid. In 10mM sodium phosphate, pH 7.2. |
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| Purity: | ≥90% (SDS-PAGE; Western blot) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Applications: | WB
in vitro Assay
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| Application Notes: | Western blot control. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1µg, Lane 3: 2µg, Lane 4: 5µg HSC70/HSP73 (bovine), (recombinant) (biotin conjugate).
Western Blot Analysis: Lane M: MW Marker, Lane 1, 3 & 5: HSC70/HSP73 (bovine), (recombinant) (biotin conjugate). Lane 2 & 6: HeLa lysate (Prod no. ADI-LYC-HL100), Lane 4: E. coli lysate. Left: probed with Hsc70 mAb (Prod. no. ADI-SPA-815); Center: probed with DnaK mAb (Prod. no. ADI-SPA-880); Right: probed with Streptavidin-AP conjugate.
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Product Literature References
Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking: D. Brown, et al. ; J. Biol. Chem.
282, 28721 (2007),
Application(s): GST Pulldown ,
Abstract;
Metabolically biotinylated adenovirus for cell targeting, ligand screening, and vector purification: M.A. Barry, et al. ; Mol. Ther.
8, 688 (2003),
Application(s): In Vitro Assay ,
Abstract;
