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HSP70-A1 (mouse), (recombinant)

ADI-SPP-502-D 50 µg 263.00 USD
ADI-SPP-502-F 200 µg 616.00 USD
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Product Details

Alternative Name:Heat shock protein 70
Recommended Dilutions/Conditions:Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
MW:~70kDa (observed)
Source:Produced in E. coli.
UniProt ID:Q61696 (Hspa1a), P17879 (Hspa1b)
Formulation:Liquid. In Dulbecco’s PBS, pH 7.5, containing 5.0mM glutathione.
Purity:≥85% (SDS-PAGE; Western blot)
Purity Detail:Purified by multi-step chromatography.
Activity assay
Application Notes:ATPase activity assay (positive). Western blot control.
Shipping:Dry Ice
Long Term Storage:-80°C
Scientific Background:The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Regulatory Status:RUO - Research Use Only
HSP70-A1 (mouse), (recombinant) SDS-PAGE
SDS-PAGE Analysis: Lane 1: MWM; Lane 2: 1.0 µg of purified Mouse Hsp70-A1 Protein.
HSP70-A1 (mouse), (recombinant) Western blot
Western Blot Analysis: Lane 1: MWM; Lane 2: 100 ng Mouse Hsp70-A1 Protein (Prod. No. ADI-SPP-502); Lane 3: 50 ng E. coli DnaK Protein (Prod. No. ADI-SPP-630); Left: probed with Hsp70 mAb (Prod. No. ADI-SPA-810) at 1.0 µg/ml. Right: probed with DnaK mAb (Prod. No. ADI-SPA-880) at 1.0 µg/ml.
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HSP70-A1 (mouse), (recombinant) SDS-PAGE HSP70-A1 (mouse), (recombinant) Western blot

Product Literature References

Impact of a Heat Shock Protein Impurity on the Immunogenicity of Biotherapeutic Monoclonal Antibodies: S.S. Rane, et al.; Pharm. Res. 36, 51 (2019), Application(s): assessment of rmHSP70 binding to aggregated mAb generated, Abstract; Full Text
Biochemical characterization of the interaction between HspA1A and phospholipids: C. McAllister, et al.; Cell Stress Chaperones 21, 41 (2016), Abstract; Full Text
Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation: V.T. Dang, et al.; PLoS One 9, e110748 (2014), Abstract; Full Text

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