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HSF1 monoclonal antibody (10H8)

ADI-SPA-950-D 50 µg 243.00 USD
ADI-SPA-950-F 200 µg 485.00 USD
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Product Details

Alternative Name:HSTF1, Heat shock factor protein 1
Immunogen:Recombinant mouse HSF1.
UniProt ID:P38532
Gene/Protein Identifier:NM_008296 (RefSeq)
Source:Purified from ascites.
Species reactivity:Human, Mouse, Rat
Monkey, Rabbit
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Predicted MW of ~60kDa. Detects a band of ~85kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.
Regulatory Status:RUO - Research Use Only
HSF1 monoclonal antibody (10H8) Immunohistochemistry
Immunohistochemistry analysis of human skeletal muscle tissue stained with HSF1, mAb (10H8) at 10µg/ml.
HSF1 monoclonal antibody (10H8) SDS-PAGE
SDS-PAGE analysis of ADI-SPA-950: Lane 1: MW Marker, Lane 2: 1µg and Lane 3: 5µg of HSF1 monoclonal antibody (10H8), resolved under partially reduced condition.
Western Blot analysis of ADI-SPA-950: Lane 1: MW Marker, Lane 2: HSF1 (human), (recombinant) (His-tag), (Prod. No. ADI-SPP-900), Lane 3: HeLa lysate, (Prod. No. ADI-LYC-HL100), Lane 4: HeLa (heat shocked) lysate, (Prod. No. ADI-LYC-HL102), Lane 5: PC-12 lysate, (Prod. No. ADI-LYC-PC100).
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HSF1 monoclonal antibody (10H8) Immunohistochemistry HSF1 monoclonal antibody (10H8) SDS-PAGE ADI-SPA-950 WB

Product Literature References

Neuroprotective effect of heat shock protein 60 on matrine-suppressed microglial activation: R. Zhang, et al.; Exp. Ther. Med. 14, 1832 (2017), Abstract; Full Text
Nox2 and Cyclosporine-Induced Renal Hypoxia: A. Djamali, et al.; Transplantation 100, 1198 (2016), Abstract; Full Text
Oxymatrine inhibits microglia activation via HSP60-TLR4 signaling: F. Ding, et al.; Biomed. Rep. 5, 623 (2016), Application(s): Western Blot, BV2 cells, Abstract; Full Text
Novel HSP90 inhibitors effectively target functions of thyroid cancer stem cell preventing migration and invasion: P.T. White, et al.; Surgery 159, 142 (2015), Application(s): Western blot, Abstract;
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): WB using mouse cell lysate, Abstract; Full Text

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