HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1
Host:
Rabbit
Immunogen:
Recombinant human HO-1.
UniProt ID:
P09601
GenBank ID:
X14782
Gene/Protein Identifier:
3162 (Entrez GeneID), 141250 (OMIM)
Source:
Purified from rabbit serum.
Species reactivity:
Human, Mouse, Rat Dog, Guinea pig, Hamster, Monkey, Rabbit, Sheep
Applications:
IHC, WB
Recommended Dilutions/Conditions:
Western Blot (1:1,000, colormetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~32kDa by Western blot.
Purity Detail:
Protein A affinity purified.
Formulation:
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
Regulatory Status:
RUO - Research Use Only
Immunohistochemistry analysis of human spleen tissue stained with HO-1, pAb at 10µg/ml.
Western blot analysis of HO-1, pAb: Lane 1: MW Marker , Lane 2: HO-1 (human), (recombinant) (Product No. ADI-SPP-732), Lane 3: Human Liver Microsomes, Lane 4: Mouse Liver Microsomes, Lane 5: Rat Liver Microsomes.
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Product Literature References
Concurrent stimulation of monocytes with CSF1 and polarizing cytokines reveals phenotypic and functional differences with classical polarized macrophages: L. An, et al.; J. Leukoc. Biol. 112, 437 (2022), Abstract;
Identification of Cytoprotective Small-Molecule Inducers of Heme-Oxygenase-1: G. Ghajar-Rahimi, et al.; Antioxidants 11, 1888 (2022), Abstract;
Dimethyl Fumarate, an Approved Multiple Sclerosis Treatment, Reduces Brain Oxidative Stress in SIV-Infected Rhesus Macaques: Potential Therapeutic Repurposing for HIV Neuroprotection: Y. Garcia-Mesa, et al.; Antioxidants 10, 416 (2021), Abstract;
Dietary lipophilic iron accelerates regional brain iron-load in C57BL6 mice: D.G. Peters, et al.; Brain Struct. Funct. 223, 1519 (2018), Abstract;
Globular adiponectin protects hepatocytes from tunicamycin-induced cell death via modulation of the inflammasome and heme oxygenase-1 induction: A. Khakurel & P.H. Park; Pharmacol. Res. 128, 231 (2018), Abstract;
Kidney injury by cyclosporine A is aggravated in heme oxygenase-1 deficient mice and involves regulation of microRNAs: A. Loboda, et al.; Acta Biochim. Pol. 65, 613 (2018), Abstract;
Pharmacological versus genetic inhibition of heme oxygenase-1 – the comparison of metalloporphyrins, shRNA and CRISPR/Cas9 system: O. Mucha, et al.; Acta Biochim. Pol. 65, 277 (2018), Abstract;
Simvastatin treatment upregulates HO-1 in patients with abdominal aortic aneurysm but independently of Nrf2: A. Piechota-Polanczyk, et al.; Oxid. Med. Cell. Longev. 2018, 2028936 (2018), Application(s): Immunofluorescence and Western-blot, Abstract; Full Text
Effect of heme oxygenase-1 on ochratoxin A-induced nephrotoxicity in mice: A. Loboda, et al.; Int. J. Biochem. Cell Biol. 84, 46 (2017), Abstract;
Nrf2 deficiency exacerbates ochratoxin A-induced toxicity in vitro and in vivo: A. Loboda, et al.; Toxicology 389, 42 (2017), Abstract;
Valproic acid downregulates heme oxygenase-1 independently of Nrf2 by increasing ubiquitination and proteasomal degradation: M. Jez, et al.; Biochem. Biophys. Res. Commun. 485, 160 (2017), Abstract;
Heme oxygenase-1 regulates mitochondrial quality control in the heart: T.D. Hull, et al.; JCI Insight 1, e85817 (2016), Abstract; Full Text
The influence of lipoic acid on caveolin-1-regulated antioxidative enzymes in the mouse model of acute ulcerative colitis: A. Piechota-Polanczyk, et al.; Biomed. Pharmacother. 84, 470 (2016), Application(s): Western blot, Abstract;
Thioredoxin Reductase Inhibition Attenuates Neonatal Hyperoxic Lung Injury and Enhances Nuclear Factor E2-Related Factor 2 Activation: Q. Li, et al.; Am. J. Respir. Cell Mol. Biol. 55, 419 (2016), Abstract; Full Text
Heat shock protein-mediated protection against Cisplatin-induced hair cell death: T.G. Baker, et al.; J. Assoc. Res. Otoryngol. 16, 67 (2015), Abstract; Full Text
Identification and Characterization of PERK Activators by Phenotypic Screening and Their Effects on NRF2 Activation: W. Xie, et al.; PLoS One 10, e0119738 (2015), Abstract; Full Text
Sodium nitrite potentiates renal oxidative stress and injury in hemoglobin exposed guinea pigs: J. H. Baek, et al.; Toxicol. 333, 89 (2015), Application(s): Western Blot using guinea pig kidney cell extracts, Abstract;
