PDI monoclonal antibody (1D3) (DyLight™ 488 conjugate)



ADI-SPA-891-488-D	50 µg	238.00 USD

ADI-SPA-891-488-F	200 µg	493.00 USD
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Product Specification

Alternative Name:	P4HB, protein disulfide isomerase

Clone:	1D3

Host:	Mouse

Isotype:	IgG1

Immunogen:	Synthetic peptide corresponding to the sequence near the C-terminus of rat PDI.

UniProt ID:	P04785

Source:	Purified from ascites

Species reactivity:	Human, Mouse, Rat Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus

Applications:	Flow Cytometry

Recommended Dilutions/Conditions:	Flow Cytometry (1:20) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~58kDa by Western blot. Cited applications include immunofluorescence.

Purity Detail:	Protein G affinity purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles. Protect from light.

Shipping:	Shipped on Blue Ice

Long Term Storage:	+4°C

Scientific Background:	The mammalian protein disulphide-isomerase (PDI) family encompasses several highly divergent proteins involved in the processing and maturation of secretory proteins in the ER by catalyzing the rearrangement of disulphide bonds. PDI, an abundant protein of the ER (>400uM), contains a carboxy-terminal retention signal sequence, KDEL, similar to that of BiP and Grp94. The PDI proteins are characterized by the presence of one or more domains of ~95-110 amino acids related to the cytoplasmic protein thioredoxin. All but the PDI-D subfamily are composed entirely of repeats of such domains, with at least one domain containing - and one domain lacking - a redox-active-Cys-X-X-Cys-tetrapeptide. In addition to roles as redox catalysts and isomerases, PDI proteins perform such functions as peptide binding and cell adhesion, and may conduct chaperone activities. Platelet surface thiols and disulphides play an important role in platelet responses. Catalytically active PDI resides on platelet surfaces where it mediates platelet aggregation and secretion by reducing disulfide bonds, thus exposing fibrinogen receptors in platelets.

Regulatory Status:	RUO - Research Use Only

PDI monoclonal antibody (1D3) (DyLight™ 488 conjugate) Flow Cytometry

Flow cytometry analysis of 10⁶ Jurkat cells stained using PDI, mAb (1D3) (DyLight™ 488 conjugate) at a concentration of 10µg/ml.

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Product Literature References

Cytoarchitecture of Zika virus infection in human neuroblastoma and Aedes albopictus cell lines: D.K. Offerdahl, et al.; Virology 15, 501 (2016), Abstract;

Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation: J.E. Kim, et al. ; PLoS One 11, e0156763 (2016), Application(s): Flow cytometric analysis, Abstract; Full Text

Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase: F. Langer, et al.; Blood 121, 2324 (2013), Application(s): Flow cytometry, Abstract; Full Text

A three-dimensional comparison of tick-borne flavivirus infection in mammalian and tick cell lines: D.K. Offerdahl, et al.; PLoS One 7, e47912 (2012), Application(s): Immunocytofluorescence, Abstract; Full Text

Cell-type specific requirements for thiol/disulfide exchange during HIV-1 entry and infection: T.S. Stantchev, et al.; Retrovirology 9, 97 (2012), Application(s): Flow cytometry, Abstract; Full Text

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