Human, Mouse, Rat Beluga, C. elegans, Chicken, Dog, E. coli, Fish, Guinea pig, Hamster, Mycobacterium leprae, Monkey, Mussel, Porcine, Rabbit, Scallop, Sheep, Water mold, Fusobacterium nucleatum
Applications:
IHC (PS), WB
Recommended Dilutions/Conditions:
Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~90kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.
Immunohistochemistry analysis of human spleen tissue stained with HSP90, mAb (AC88) at 10µg/ml.
Western blot analysis of HSP90, mAb (AC88) (Prod. No. ADI-SPA-830): Lane 1: MW Marker; Lane 2: HSP90 (human), (native) (Prod. No. ADI-SPP-770); Lane 3: HSP90β, (human) (recombinant) (Prod. No. ADI-SPP-777); Lane 4: HSP90α (human), (recombinant) (Prod. No. ADI-SPP-776); Lane 5: HeLa (heat shocked) (Prod. No. ADI-LYC-HL101); Lane 6: 3T3 (heat shocked) (Prod. No. ADI-LYC-3T101; Lane 7: PC-12 (heat shocked) (Prod. No. ADI-LYC-PC101); Lane 8: CHO-K1 (heat shocked).
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Product Literature References
Aging Is Associated With Impaired Postprandial Response of Skeletal Muscle Protein Synthesis to High-Intensity Muscle Contraction in Mice: S. Ato, et al.; J. Gerontol. A Biol. Sci. Med. Sci. 78, 587 (2023), Abstract;
Azaphilones produced by Penicillium maximae with their cell death-inducing activity on Adriamycin-treated cancer cell: T. Matsumoto, et al.; Genes Environ. 45, 5 (2023), Abstract;
Novel hydroxamic acid derivative induces apoptosis and constrains autophagy in leukemic cells: M.A. Fischer, et al.; J. Adv. Res. (2023), Abstract;
A novel C-terminal heat shock protein 90 inhibitor that overcomes STAT3-Wnt-β-catenin signaling-mediated drug resistance and adverse effects: H.J. Lee, et al.; Theranostics 12, 105 (2022), Abstract;
Computational identification of new potential transcriptional partners of ERRα in breast cancer cells: specific partners for specific targets: C. Cerutti, et al.; Sci. Rep. 12, 3826 (2022), Abstract;
Improvement in heat stress-induced multiple organ dysfunction and intestinal damage through protection of intestinal goblet cells from prostaglandin E1 analogue misoprostol: H.P. Hii, et al.; Life Sci. 310, 121039 (2022), Abstract;
The epigenetic modifier HDAC2 and the checkpoint kinase ATM determine the responses of microsatellite instable colorectal cancer cells to 5-fluorouracil: N. Kiweler, et al.; Cell Biol. Toxicol. (2022), Abstract;
Therapeutic Effect of Acer tegmentosum Maxim Twig Extract in Bile Duct Ligation-Induced Acute Cholestasis in Mice: U.J. Bae, et al.; J. Med. Food 25, 652 (2022), Abstract;
Assessing BRCA1 activity in DNA damage repair using human induced pluripotent stem cells as an approach to assist classification of BRCA1 variants of uncertain significance: M. Ozgencil, et al.; PLoS One 16, e0260852 (2021), Abstract;
Chemical structures and induction of cell death via heat shock protein inhibition of the prenylated phloroglucinol derivatives isolated from Hypericum erectum: T. Μatsumoto, et al.; Fitoterapia 156, 105097 (2021), Abstract;
Loss of Sirt6 in adipocytes impairs the ability of adipose tissue to adapt to intermittent fasting: D. Wu, et al.; Exp. Mol. Med. 1038, 12276 (2021), Abstract;
Polymer-ritonavir derivate nanomedicine with pH-sensitive activation possesses potent anti-tumor activity in vivo via inhibition of proteasome and STAT3 signaling: L. Sivák, et al.; J. Control Release 332, 563 (2021), Application(s): Flow Cytometry on CT26 cells, Abstract;
Urocortin 3 overexpression reduces ER stress and heat shock response in 3T3-L1 adipocytes: S. Kavalakatt, et al.; Sci. Rep. 11, 15666 (2021), Abstract;
A novel function of FAF1, which induces dopaminergic neuronal death through cell-to-cell transmission: G. Park, et al.; Cell Commun. Signal. 18, 133 (2020), Abstract; Full Text
Histone deacetylase inhibitors dysregulate DNA repair proteins and antagonize metastasis-associated processes: N. Kiweler, et al.; J. Cancer Res. Clin. Oncol. 146, 343 (2020), Application(s): Western-blot using Renca cells, Abstract; Full Text
Luteolin prevents liver from tunicamycin-induced endoplasmic reticulum stress via nuclear factor erythroid 2-related factor 2-dependent sestrin 2 induction: K.H. Jegal, et al.; Toxicol. Appl. Pharmacol. 399, 115036 (2020), Application(s): Western-blot using HepG2 cells, Abstract;
Proteostasis regulators as potential rescuers of PMM2 activity: A. Vilas, et al.; Biochim. Biophys. Acta Mol. Basis Dis. 1866, 165777 (2020), Application(s): Western-blot using patient-derived skin fibroblasts, Abstract;
A novel anti-HER2 anthracycline-based antibody-drug conjugate induces adaptive anti-tumor immunity and potentiates PD-1 blockade in breast cancer: L. D'Amico, et al.; J. Immunother. Cancer 7, 16 (2019), Abstract; Full Text
A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers: A. Nigro, et al.; Methods Mol. Biol. 1884, 317 (2019), Abstract;
Cocaine-induced endocannabinoid signaling mediated by sigma-1 receptors and extracellular vesicle secretion: Y. Nakamura, et al.; Elife 8, e47209 (2019), Application(s): Western-blot using mouse brain lysates, Abstract; Full Text
Human platelet lysate as validated replacement for animal serum to assess chemosensitivity: M. Pons, et al.; ALTEX 36, 277 (2019), Abstract;
Modeling of LMNA-Related Dilated Cardiomyopathy Using Human Induced Pluripotent Stem Cells: D. Shah, et al.; Cells 8, 594 (2019), Abstract; Full Text
The EGFR exon 19 mutant L747-A750> P exhibits distinct sensitivity to tyrosine kinase inhibitors in lung adenocarcinoma: A. Truini, et al.; Clin. Cancer Res. 25, 6382 (2019), Application(s): Western-blot using CHO cells, Abstract; Full Text
Establishment, functional and genetic characterization of a colon derived large cell neuroendocrine carcinoma cell line: M. Gock, et al.; World J. Gastroenterol. 24, 3749 (2018), Abstract; Full Text
Establishment, functional and genetic characterization of three novel patient-derived rectal cancer cell lines: M. Glock, et al.; World Journal of Gastroenterology 24, 4880 (2018), Application(s): Western blot , Abstract; Full Text
Loss of Wilms tumor 1 protein is a marker for apoptosis in response to replicative stress in leukemic cells: M. Pons, et al.; Arch. Toxicol. 92, 2119 (2018), Abstract;
Osterix regulates corticalization for longitudinal bone growth via integrin β3 expression: Y.J. Moon, et al.; Exp. Mol. Med. 50, 80 (2018), Abstract; Full Text
Revisiting the putative role of heme as a trigger of inflammation: F. Vallelian, et al.; Pharmacol. Res. Perspect. 6, e00392 (2018), Abstract; Full Text
Skeletal Muscle-Specific Overexpression of Heat Shock Protein 72 Improves Skeletal Muscle Insulin-Stimulated Glucose Uptake But Does Not Alter Whole Body Metabolism: J. Marshall, et al.; Diabetes Obes. Metab. 20, 1928 (2018), Abstract; Full Text
Targeting of drug-loaded nanoparticles to tumor sites increases cell death and release of danger signals: M. Alev, et al.; J. Control Release 285, 67 (2018), Abstract;
The histone deacetylases HDAC1 and HDAC2 are required for the growth and survival of renal carcinoma cells: N. Kiweler, et al.; Arch. Toxicol. 92, 2227 (2018), Abstract;
ATAT1 is essential for regulation of homeostasis-retaining cellular responses in corticotrophs along hypothalamic-pituitary-adrenal axis: T. Nakakura, et al.; Cell Tissue Res. 370, 169 (2017), Abstract;
Class I histone deacetylases regulate p53/NF-κB crosstalk in cancer cells: C. Schafer, et al.; Cell. Signal. 29, 218 (2017), Abstract;
Docosahexaenoic acid (DHA) promotes immunogenic apoptosis in human multiple myeloma cells, induces autophagy and inhibits STAT3 in both tumor and dendritic cells: D. D'Eliseo, et al.; Genes Cancer 8, 426 (2017), Abstract; Full Text
How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot: M. Beyer, et al.; Methods Mol. Biol. 1510, 355 (2017), Abstract;
HSP90 is necessary for the ACK1-dependent phosphorylation of STAT1 and STAT3: N. Mahendrarajah, et al.; Cell. Signal. 39, 9 (2017), Abstract;
Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes: M.J. Schonenberger, et al.; Methods Mol. Biol. 1595, 13 (2017), Abstract;
Overexpression of heat-shock factor 1 is associated with a poor prognosis in esophageal squamous cell carcinoma: Y. Tsukao, et al.; Oncol. Lett. 13, 1819 (2017), Abstract; Full Text
Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease: P. Luningschror, et al.; Nat. Commun. 8, 678 (2017), Abstract; Full Text
The ubiquitin conjugating enzyme Ube2W regulates solubility of the Huntington's disease protein, huntingtin: B. Wang, et al.; Neurobiol. Dis. 109, 127 (2017), Application(s): Western Blot, Abstract;
Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90: G. Zhang, et al.; Nat. Commun. 8, 589 (2017), Abstract; Full Text
Acidosis Acts through HSP90 in a PHD/VHL-Independent Manner to Promote HIF Function and Stem Cell Maintenance in Glioma: A. Filatova, et al.; Cancer Res. 76, 5845 (2016), Abstract;
Apratoxin A Shows Novel Pancreas-Targeting Activity through the Binding of Sec 62: K.C. Huang, et al.; Mol. Cancer Ther. 15, 1208 (2016), Abstract;
Different growth and metastatic phenotypes associated with a cell-intrinsic change of Met in metastatic melanoma: E. Adachi, et al.; Oncotarget 7, 70779 (2016), Abstract; Full Text
Dihydropyridine Derivatives Modulate Heat Shock Responses and have a Neuroprotective Effect in a Transgenic Mouse Model of Alzheimer's Disease: A. Kasza, et al.; J. Alzheimers Dis. 53, 557 (2016), Abstract; Full Text
Histone deacetylase inhibitors induce proteolysis of activated CDC42-associated kinase-1 in leukemic cells: N. Mahendrarajah, et al.; J. Cancer Res. Clin. Oncol. 142, 2263 (2016), Abstract;
miR-135a Inhibits the Invasion of Cancer Cells via Suppression of ERRα: V. Tribollet, et al.; PLoS One 11, e0156445 (2016), Abstract; Full Text
Nuclear COMMD1 Is Associated with Cisplatin Sensitivity in Ovarian Cancer: A. Fedoseienko, et al.; PLoS One 11, e0165385 (2016), Abstract; Full Text
Proinsulin and HSP90 as Biomarkers of Beta Cell Stress in the Early Period after Onset of Type 1 Diabetes: R.A. Watkins, et al.; Transl. Res. 168, 96 (2016), Application(s): Immunoblot Analysis, Abstract;
β-Arrestin-2 Counters CXCR7-Mediated EGFR Transactivation and Proliferation: G. Kallifatidis, et al.; Mol. Cancer Res. 14, 493 (2016), Abstract; Full Text
β-Secretase 1's Targeting Reduces Hyperphosphorilated Tau, Implying Autophagy Actors in 3xTg-AD Mice: D. Piedrahita, et al.; Front Cell Neurosci. 9, 498 (2016), Application(s): Western blot, Abstract; Full Text
Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin: A.M. Dudek-Peric, et al.; Cancer Res. 75, 1603 (2015), Abstract;
Concurrent MEK and autophagy inhibition is required to restore cell death associated danger-signalling in Vemurafenib-resistant melanoma cells: S. Martin, et al.; Biochem. Pharmacol. 93, 290 (2015), Application(s): Flow cytometry using human melanoma cell lines A375, 451-LU, 451-LU/RES, M1617 and M1617/RES, Abstract;
Functional-genetic dissection of HDAC dependencies in mouse lymphoid and myeloid malignancies: G.M. Matthews, et al.; Blood 126, 2392 (2015), Abstract; Full Text
Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE: K. Saito, et al.; Eur. J. Immunol. 45, 2028 (2015), Abstract; Full Text
Potential Prognostic Value of Histone Deacetylase 6 and Acetylated Heat-Shock Protein 90 in Early-Stage Breast Cancer : Y. Park, et al.; J. Breast Cancer 18, 249 (2015), Application(s): Immunohistochemical analysis, Abstract; Full Text
Targeting the hsp70 gene delays mammary tumor initiation and inhibits tumor cell metastasis: J. Gong, et al.; Oncogene 34, 5640 (2015), Application(s): Western Blotting, Abstract;
Estrogen-related receptor α decreases RHOA stability to induce orientated cell migration: J. Sailland, et al.; PNAS 111, 15108 (2014), Application(s): Western blot using human MDA-MB231 mammary epithelial cells and human PC3 prostate cancer cells, Abstract; Full Text
A dual role for Hdac1: oncosuppressor in tumorigenesis, oncogene in tumor maintenance: F. Santoro, et al.; Blood 121, 3459 (2013), Abstract;
OLA1 protects cells in heat shock by stabilizing HSP70: R.F. Mao, et al.; Cell Death Dis. 4, e491 (2013), Application(s): Immunoprecipitation using human HEK-293T and MDA-MB-231 cells, Abstract; Full Text
Secreted Hsp90 is a novel regulator of the epithelial to mesenchymal transition (EMT) in prostate cancer: M.W. Hance, et al.; J. Biol. Chem. 287, 37732 (2012), Abstract; Full Text
Measuring the pharmacodynamic effects of a novel Hsp90 inhibitor on HER2/neu expression in mice using Zr-DFO-trastuzumab: J.P. Holland, et al.; PLoS One 5, e8859 (2010), Abstract; Full Text
Effects of neurotoxic insecticides on heat-shock proteins and cytokine transcription in Chinook salmon (Oncorhynchus tshawytscha): I. Werner, et al. ; Ecotoxicol. Environ. Saf. 72, 182 (2009), Application(s): WB using fish tissue, Abstract;
Bovine serum amine oxidase and spm potentiate docetaxel and interferon-α effects in inducing apoptosis on human cancer cells through the generation of oxidative stress: M. Caraglia, et al. ; Biochim. Biophys. Acta 1783, 2269 (2008), Application(s): WB using human cell lysates, Abstract;
CHIP deletion reveals functional redundancy of E3 ligases in promoting degradation of both signaling proteins and expanded glutamine proteins: A. Lieberman, et al. ; Hum. Mol. Genet. 17, 3942 (2008), Application(s): WB using mouse cell lysates, Abstract;
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases: S. Chen & I.R. Brown; Cell Stress Chaperones 12, 51 (2007), Application(s): WB, IF using rat tissue, Abstract;
Yeast is selectively hypersensitised to heat shock protein90 (Hsp90)-targetting drugs with heterologous expression of the human Hsp90beta, a property that can be exploited in screens for new Hsp90 chaperone inhibitors: C. Prodromou, et al. ; Gene 302, 165 (2003), Application(s): WB using human & C. elegans samples, Abstract;
Intermittent hypoxia improves atrial tolerance to subsequent anoxia and reduces stress protein expression: D.J. Paterson, et al. ; Acta Physiol. Scand. 172, 89 (2001), Application(s): WB using guinea pig samples, Abstract;
Involvement of heat shock protein90 in the degradation of mutant insulin receptors by the proteasome: M. Kobayashi, et al. ; J. Biol. Chem. 273, 11183 (1998), Application(s): WB, ICC, IP using monkey samples, Abstract;
Protein phosphatase 5 is a major component of glucocorticoid receptor.hsp90 complexes with properties of an FK506-binding immunophilin: W.B. Pratt, et al. ; J. Biol. Chem. 272, 16224 (1997), Application(s): WB using mouse & human samples, Abstract;
Immunological evidence that the nonhormone binding component of avian steroid receptors exists in a wide range of tissues and species: D.O. Toft, et al. ; Biochemistry 24, 6586 (1985), Application(s): IP using chicken, mouse, rat, porcine & water mold samples, Abstract;
