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KDEL monoclonal antibody (10C3)

ADI-SPA-827-D 50 µg 210.00 USD
ADI-SPA-827-F 200 µg 443.00 USD
ADI-SPA-827-J 1 mg 1,694.00 USD
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Product Specification

Alternative Name:Lys-Asp-Glu-Leu, Lysine-aspartic acid-glutamate-leucine
Immunogen:Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78.
UniProt ID:P06761
GenBank ID:M14050
Source:Purified from ascites.
Species reactivity:Avian, Insect, Mammalian, Plant, Yeast
Specificity:Recognizes proteins containing the KDEL sequence.
Applications:ELISA, Flow Cytometry, ICC, IF, IHC (PS), IP, WB
Electron microscopy
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot..
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
KDEL monoclonal antibody (10C3) Western blot
Western blot analysis: Lane 1: Grp78 (BiP) recombinant protein (Prod No. ADI-SPP-765), Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked) (Prod No. ADI-LYC-HL101).
KDEL monoclonal antibody (10C3) Immunofluorescence
Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).
KDEL monoclonal antibody (10C3) Immunohistochemistry
Immunohistochemistry analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10µg/ml.
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KDEL monoclonal antibody (10C3) Western blot KDEL monoclonal antibody (10C3) Immunofluorescence KDEL monoclonal antibody (10C3) Immunohistochemistry

Product Literature References

Decomposition profile data analysis of multiple drug effects identifies endoplasmic reticulum stress-inducing ability as an unrecognized factor: K. Morita, et al.; Sci. Rep. 10, 13139 (2020), Abstract; Full Text
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation: T. Yamanaka, et al.; Sci. Rep. 10, 7610 (2020), Abstract; Full Text
The novel missense mutation Met48Lys in FKBP22 changes its structure and functions: Y. Ishikawa, et al.; Sci. Rep. 10, 497 (2020), Abstract; Full Text
Thyrocyte cell survival and adaptation to chronic endoplasmic reticulum stress due to misfolded thyroglobulin: Y. Morishita, et al.; J. Biol. Chem. (2020), Abstract; Full Text
Efficient RNA interference-based knockdown of mutant torsinA reveals reversibility of PERK-eIF2α pathway dysregulation in DYT1 transgenic rats in vivo: G. Beauvais, et al.; Brain Res. 1706, 24 (2019), Abstract;
IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages: E.A. Ayaub, et al.; Immunol. Cell Biol. 97, 203 (2019), Abstract;
Pharmacologic ATF6 activation confers global protection in widespread disease models by reprograming cellular proteostasis: E. A. Blackwood, et al.; Nat. Commun. 10, 187 (2019), Abstract; Full Text
Proinsulin misfolding is an early event in the progression to type 2 diabetes: A. Arunagiri, et al.; Elife 8, e44532 (2019), Abstract; Full Text
Proper secretion of the serpin antithrombin relies strictly on thiol-dependent quality control: B.M. Adams, et al.; J. Biol. Chem. (2019), Abstract; Full Text
Schwann cells ER-associated degradation contributes to myelin maintenance in adult nerves and limits demyelination in CMT1B mice: V.G. Volpi, et al.; PLoS Genet. 15, e1008069 (2019), Abstract; Full Text
The lysosomal transporter MFSD1 is essential for liver homeostasis and critically depends on its accessory subunit GLMP: D. Massa Lopez, et al.; Elife 8, e50025 (2019), Abstract; Full Text
The Multifunctional Sorting Protein PACS-2 Controls Mitophagosome Formation in Human Vascular Smooth Muscle Cells through Mitochondria-ER Contact Sites: M. Moulis, et al.; Cells 8, 638 (2019), Abstract; Full Text
Characterization of Non-amyloidogenic G101S Transthyretin: Y. Wakita, et al.; Biol. Pharm. Bull. 41, 628 (2018), Abstract;
Exploring the Interaction Between eIF2α Dysregulation, Acute Endoplasmic Reticulum Stress and DYT1 Dystonia in the Mammalian Brain: G. Beauvais, et al.; Neuroscience 371, 455 (2018), Abstract;
Laminar Flow Inhibits ER Stress-Induced Endothelial Apoptosis through PI3K/Akt-Dependent Signaling Pathway: S. Kim, et al.; Mol. Cells 41, 964 (2018), Abstract; Full Text
Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response: P. Gomez-Fernandez, et al.; Front. Immunol. 9, 2934 (2018), Abstract; Full Text
Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained: P. Lebeau, et al.; J. Biol. Chem. 293, 7329 (2018), Abstract;
Nitazoxanide inhibits paramyxovirus replication by targeting the Fusion protein folding: role of glycoprotein-specific thiol oxidoreductase ERp57: S. Piacentini, et al.; Sci. Rep. 8, 10425 (2018), Abstract; Full Text
Rab44, a novel large Rab GTPase, negatively regulates osteoclast differentiation by modulating intracellular calcium levels followed by NFATc1 activation: Y. Yamaguchi, et al.; Cell. Mol. Life Sci. 75, 33 (2018), Abstract;
The BAX/BAK-like protein BOK is a prognostic marker in colorectal cancer: S. Carberry, et al.; Cell Death Dis. 9, 125 (2018), Application(s):Western Blot, Abstract; Full Text
UPR activation specifically modulates glutamate neurotransmission in the cerebellum of a mouse model of autism: L. Trobiani, et al.; Neurobiol. Dis. 120, 139 (2018), Abstract;
BRAF-mutated cells activate GCN2-mediated integrated stress response as a cytoprotective mechanism in response to vemurafenib: I. Nagasawa, et al.; Biochem. Biophys. Res. Commun. 482, 1491 (2017), Abstract;
Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A: J. Zhang, et al.; J. Biol. Chem. 292, 10328 (2017), Abstract; Full Text
Disruption of the mitochondria-associated ER membrane (MAM) plays a central role in palmitic acid-induced insulin resistance: S. Shinjo, et al.; Exp. Cell Res. 359, 86 (2017), Abstract;
eIF2α phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet: W.G. Choi, et al.; Nutr. 14, 48 (2017), Abstract; Full Text
ER Stress Induced by Tunicamycin Triggers α-Synuclein Oligomerization, Dopaminergic Neurons Death and Locomotor Impairment: a New Model of Parkinson's Disease: V. Coppola-Segovia, et al.; Mol. Neurobiol. 54, 5798 (2017), Abstract;
Human neutrophil elastase induces endothelial cell apoptosis by activating the PERK-CHOP branch of the unfolded protein response: I. Grechowa, et al.; FASEB J. 31, 3868 (2017), Abstract;
Ligand and Target Discovery by Fragment-Based Screening in Human Cells: C.G. Parker, et al.; Cell 168, 527 (2017), Abstract; Full Text
Mutant uromodulin expression leads to altered homeostasis of the endoplasmic reticulum and activates the unfolded protein response: C. Schaeffer, et al.; PLoS One 12, e0175970 (2017), Abstract; Full Text
Pofut1 point-mutations that disrupt O-fucosyltransferase activity destabilize the protein and abolish Notch1 signaling during mouse somitogenesis: R. Ajima, et al.; PLoS One 12, e0187248 (2017), Application(s): IHC, Abstract; Full Text
Sublethal endoplasmic reticulum stress caused by the mutation of immunoglobulin heavy chain-binding protein induces the synthesis of a mitochondrial protein, pyrroline-5-carboxylate reductase 1: H. Jin, et al.; Cell Stress Chaperones 22, 77 (2017), Abstract; Full Text
Trimethylamine-N-oxide, as a risk factor for atherosclerosis, induces stress in J774A.1 murine macrophages: A. Mohammadi, et al.; Adv. Med. Sci. 63, 57 (2017), Application(s): Western Blot, Abstract;
A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargos: H.C. Tie, et al.; Mol. Biol. Cell 27, 848 (2016), Application(s): Tagging on the Golgi, Abstract; Full Text
Activation of the unfolded protein response promotes axonal regeneration after peripheral nerve injury: M. Onate, et al.; Sci. Rep. 6, 21709 (2016), Application(s): Histological analysis, Abstract; Full Text
Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system: L. Ulbrich, et al.; J. Biochem. 473, 423 (2016), Abstract; Full Text
Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion: M. Ueda, et al.; Biochem. Biophys. Res. Commun. 474, 259 (2016), Abstract;
High expression of α-synuclein in damaged mitochondria with PLA2G6 dysfunction: H. Sumi-Akamaru, et al.; Acta. Neuropathol. Commun. 4, 27 (2016), Application(s): Immunohistochemistry, Abstract; Full Text
Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells: Y. Niwa, et al.; Mol. Biol. Cell 27, 744 (2016), Application(s): Immunofluorescence, Abstract; Full Text
Morphology and Molecular Composition of Purified Bovine Viral Diarrhea Virus Envelope: N. Callens, et al.; PLoS One 12, e1005476 (2016), Application(s): Western blot, Abstract; Full Text
mTOR Hyperactivation by Ablation of Tuberous Sclerosis Complex 2 in the Mouse Heart Induces Cardiac Dysfunction with the Increased Number of Small Mitochondria Mediated through the Down-Regulation of Autophagy: M. Taneike, et al.; PLoS One 11, e0152628 (2016), Abstract; Full Text
Mutations in REEP6 Cause Autosomal-Recessive Retinitis Pigmentosa: G. Arno, et al.; Am. J. Hum. Genet. 99, 1305 (2016), Abstract; Full Text
T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation: R.P. Laura, et al.; PLoS One. 11, e0149391 (2016), Application(s): Western blot, Abstract; Full Text
A novel UGGT1 and p97-dependent checkpoint for native ectodomains with ionizable intramembrane residue: J. Merulla, et al.; Mol. Biol. Cell 26, 1532 (2015), Abstract; Full Text
A53T Human α-Synuclein Overexpression in Transgenic Mice Induces Pervasive Mitochondria Macroautophagy Defects Preceding Dopamine Neuron Degeneration: L. Chen, et al.; J. Neurosci. 35, 890 (2015), Application(s): Immunofluorescence, Abstract;
Cellular localization and tissue distribution of endogenous DFCP1 protein: T. Nanao, et al.; Biomed. Res. 36, 121 (2015), Application(s): Immunocytochemistry, Abstract; Full Text
CHOP deficiency prevents methylglyoxal-induced myocyte apoptosis and cardiac dysfunction: D.H. Nam, et al.; J. Mol. Cell. Cardiol. 85, 168 (2015), Abstract;
Endogenously tagged rab proteins: a resource to study membrane trafficking in Drosophila: S. Dunst, et al.; Dev. Cell. 33, 351 (2015), Application(s): Immunohistochemistry, Abstract;
ER stress induces SIRT1 expression via the PI3K-Akt-GSK3β signaling pathway and promotes hepatocellular injury: T. Koga, et al.; J. Biol. Chem. 290, 10143 (2015), Application(s): Western Blot, Abstract;
FAD-linked Presenilin-1 V97L mutation impede tranport regulation and intracellular Ca2+ homeostasis under ER stress: Y. Shao, et al.; Int. J. Clin. Exp. Med. 8, 20742 (2015), Application(s): Immunoblot analysis, Abstract; Full Text
Gefitinib and Erlotinib Lead to Phosphorylation of Eukaryotic Initiation Factor 2 Alpha Independent of Epidermal Growth Factor Receptor in A549 Cells: S. Koyama, et al.; PLoS One 10, e0136176 (2015), Abstract; Full Text
GSK-3β-dependent downregulation of γ-taxilin and αNAC merge to regulate ER stress responses: Y. Hotokezaka, et al.; Cell Death Dis. 6, e1719 (2015), Application(s): Western Blot, Abstract; Full Text
Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE: K. Saito, et al.; Eur. J. Immunol. 45, 2028 (2015), Abstract; Full Text
Pancreas-Specific Sirt1-Deficiency in Mice Compromises Beta-Cell Function without Development of Hyperglycemia: A.V. Pinho, et al.; PLoS One 10, e0128012 (2015), Application(s): Immunofluorescence, Abstract; Full Text
PINK1 and Parkin Control Localized Translation of Respiratory Chain Component mRNAs on Mitochondria Outer Membrane: S. Gehrke, et al.; Cell Metab. 21, 95 (2015), Application(s): Western Blotting, Abstract;
The serine protease hepsin mediates urinary secretion and polymerisation of Zona Pellucida domain protein uromodulin: M. Brunati, et al.; Elife 4, e08887 (2015), Abstract; Full Text
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol. 97, 518 (2015), Application(s): Western Blot, Abstract;
Activation of the Proapoptotic Unfolded Protein Response in Plaques of the Human Carotid Artery: B. Dorweiler, et al.; Eur. J. Vasc. Endovasc. Surg. 48, 248 (2014), Application(s): Immunohistochemistry using formalin-fixed paraffin-embedded human carotid artery tissue samples, Abstract;
Caveolin 1 is critical for abdominal aortic aneurysm formation induced by angiotensin II and inhibition of lysyl oxidase: T. Takayanagi, et al.; Clin. Sci. (Lond.) 126, 785 (2014), Abstract; Full Text
Alteration of endoplasmic reticulum lipid rafts contributes to lipotoxicity in pancreatic β-cells: E. Boslem, et al.; J. Biol. Chem. 288, 26569 (2013), Application(s): Immunocytochemistry and Western blot using murine pancreatic islet and MIN6 cells, Abstract; Full Text
Down-Regulation of Survivin by Nemadipine-A Sensitizes Cancer Cells to TRAIL-Induced Apoptosis: S. Park, et al.; Biomol. Ther. 21, 29 (2013), Abstract; Full Text
The unfolded protein response to endoplasmic reticulum stress in cultured astrocytes and rat brain during experimental diabetes: K.R. Lind, et al.; Neurochem. Int. 62, 784 (2013), Abstract; Full Text
Analysis of the endoplasmic reticulum subproteome in the livers of type 2 diabetic mice: E.C. Park, et al.; Int. J. Mol. Sci. 13, 17230 (2012), Abstract; Full Text
Mesencephalic astrocyte-derived neurotrophic factor protects the heart from ischemic damage and is selectively secreted upon sarco/endoplasmic reticulum calcium depletion: C.C. Glembotski, et al.; J. Biol. Chem. 287, 25893 (2012), Abstract; Full Text
Endoplasmic Reticulum Stress Induces Leptin Resistance: K. Ozawa, et al. ; Mol. Pharmacol. 74, 1610 (2008), Application(s): WB using mouse & human cell lysates, Abstract;
Nedd4 Family-interacting Protein 1 (Ndfip1) Is Required for the Exosomal Secretion of Nedd4 Family Proteins: S. Tan, et al. ; J. Biol. Chem. 283, 32621 (2008), Application(s): IF using human tissue culture, Abstract;
Pofut1 is required for the proper localization of the Notch receptor during mouse development: Y. Saga, et al. ; Mech. Dev. 125, 663 (2008), Application(s): IF using mouse tissue, Abstract;
The Hepatitis B Virus Precore Protein Is Retrotransported from Endoplasmic Reticulum (ER) to Cytosol through the ER-associated Degradation Pathway: D. Sitterlin, et al. ; J. Biol. Chem. 283, 32352 (2008), Application(s): IP using human cell lysates, Abstract;
Molecular pathogenesis of seipin/BSCL2-related motor neuron diseases: D. Ito, et al. ; Ann. Neurol. 61, 237 (2007), Application(s): WB, ICC, IF, IHC using human & mouse cell lysates & tissue, Abstract;
Tissue and subcellular distribution of CLIC1: B. Ulmasov, et al.; BMC Cell Biol. 8, 8 (2007), Abstract;
Boca, an endoplasmic reticulum protein required for wingless signaling and trafficking of LDL receptor family members in Drosophila: J. Culi & R.S. Mann; Cell 112, 343 (2003), Abstract;
Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity: R.C. Austin, et al. ; J. Biol. Chem. 278, 17438 (2003), Application(s): WB using human samples, Abstract;
The amyloid precursor protein protects PC12 cells against endoplasmic reticulum stress-induced apoptosis: H.M. Prehn, et al. ; J. Neurochem. 87, 248 (2003), Application(s): WB using rat samples, Abstract;
A protein sequence that can encode native structure by disfavoring alternate conformations: W.C. Wigley, et al.; Nat. Struct. Biol. 9, 381 (2002), Abstract;
Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae: A.S. Robinson, et al. ; Biotechnol. Prog. 18, 942 (2002), Application(s): WB using yeast samples, Abstract;
Decreased protein expression and intermittent recoveries in BiP levels result from cellular stress during heterologous protein expression in Saccharomyces cerevisiae: K.J. Kauffman, et al.; Biotechnol. Prog. 18, 942 (2002), Abstract;
Developmental regulation of neuronal K(Ca) channels by TGFbeta1: an essential role for PI3 kinase signaling and membrane insertion: S.E. Dryer, et al. ; J. Neurophysiol. 88, 954 (2002), Application(s): ICC using chicken samples, Abstract;
Efficacy of plant-produced recombinant antibodies against HCG: R. Finnern, et al. ; Hum. Reprod. 17, 2054 (2002), Application(s): WB, EIA using plant (tobacco) samples, Abstract;
Efficacy of plant-produced recombinant antibodies against HCG: S. Kathuria, et al.; Hum. Reprod. 17, 2054 (2002), Abstract;
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum: N. Shastri, et al. ; Nature 419, 480 (2002), Application(s): WB, ICC using monkey samples, Abstract;
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum: T. Serwold, et al.; Nature 419, 480 (2002), Abstract;
Mass transport of proform of a KDEL-tailed cysteine proteinase (SH-EP) to protein storage vacuoles by endoplasmic reticulum-derived vesicle is involved in protein mobilization in germinating seeds: K. Toyooka, et al.; J. Cell Biol. 148, 453 (2000), Abstract;
Identification of amino acids in the binding pocket of the human KDEL receptor: A.A. Scheel & H.R. Pelham; J. Biol. Chem. 273, 2467 (1998), Abstract;
Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins: A. Choukhi, et al.; J. Virol. 72, 3851 (1998), Abstract;
Mutants of the yeast Yarrowia lipolytica defective in protein exit from the endoplasmic reticulum are also defective in peroxisome biogenesis: V.I. Titorenko & R.A. Rachubinski; Mol. Cell Biol. 18, 2789 (1998), Abstract;
Identification by anti-idiotype antibodies of an intracellular membrane protein that recognizes a mammalian endoplasmic reticulum retention signal: D. Vaux, et al.; Nature 345, 495 (1990), Abstract;
The retention signal for soluble proteins of the endoplasmic reticulum: H.R. Pelham; Trends Biochem. Sci. 15, 483 (1990), Abstract;

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