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HSC70/HSP73 monoclonal antibody (1B5) (biotin conjugate)

ADI-SPA-815B-D 50 µg 270.00 USD
ADI-SPA-815B-F 200 µg 572.00 USD
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Product Details

Alternative Name:Heat shock protein 70, Hsc70, Hsp73
Immunogen:Native hamster Hsc70 (Hsp73).
UniProt ID:P19378
GenBank ID:M34561
Source:Purified from ascites.
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
Applications:ELISA, Flow Cytometry, ICC, IHC, IP, WB
Electron microscopy
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~73kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Use/Stability:+4°C for short term storage; -20°C for long term storage; Avoid Freeze/Thaw cycles.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Regulatory Status:RUO - Research Use Only
HSC70/HSP73 monoclonal antibody (1B5) (biotin conjugate) Western blot
Western blot analysis of HSC70/HSP73, mAb (1B5) (biotin conjugate) (Prod. No. ADI-SPA-815B): Lane 1: HSC70/HSP73 (bovine), (rec) (Prod. No. ADI-SPP-751); Lane 2: HSP70/HSP72 (human), (recombinant) (Prod. No. ADI-NSP-555); Lane 3: 3T3 (heat shocked) (Prod. No. ADI-LYC-3T101); Lane 4: PC-12 (heat shocked) (Prod. No. ADI-LYC-PC101); Lane 5: CHO-K1 (heat shocked); Lane 6: GPC-16 (heat shocked); Lane 7: HeLa (heat shocked) (Prod. No. ADI-LYC-HL101); Lane 8: Vero (heat shocked).
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HSC70/HSP73 monoclonal antibody (1B5) (biotin conjugate) Western blot

Product Literature References

HLA-B influences integrin beta-1 expression and pancreatic cancer cell migration: B.H. Sliker, et al.; Exp. Cell. Res. 390, 111960 (2020), Application(s): Western-blot using pancreatic cancer cell lines, Abstract;

General Literature References

HSC70 blockade by the therapeutic peptide P140 affects autophagic processes and endogenous MHCII presentation in murine lupus: N. Page, et al.; Ann. Rheum. Dis. 70, 837 (2011), Application(s): FACS, Abstract; Full Text
The Mammalian Disaggregase Machinery: Hsp110 Synergizes with Hsp70 and Hsp40 to Catalyze Protein Disaggregation and Reactivation in a Cell-Free System: J. Shorter; PLoS One 6, e26319 (2011), Application(s): Western Blot, Abstract; Full Text

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