Replaces Prod. #: ALX-804-068
Product Details
| Alternative Name: | HspB1, Heat shock protein 27 |
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| Clone: | G3.1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Native human Hsp27. |
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| UniProt ID: | P04792 |
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| GenBank ID: | L39370 |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Fish, Monkey
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| Applications: | ELISA, ICC, IHC (PS), IP, WB
Electron microscopy, in vitro Assay
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~27kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human skeletal muscle tissue stained with HSP27, mAb (G3.1) at 10µg/ml.
Western blot analysis of HSP27, mAb (G3.1) (Prod. No. ADI-SPA-800). Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: Vero (heat shocked), Lane 4: HSP27 (human) (recombinant) (Prod. No. ADI-SPP-715).
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Product Literature References
Omptin Proteases of Enterobacterales Show Conserved Regulation by the PhoPQ Two-Component System but Exhibit Divergent Protection from Antimicrobial Host Peptides and Complement: Y.H. Cho, et al.; Infect. Immun.
91, e00518 (2023),
Abstract;
A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins: T.R. Alderson , et al.; EMBO J.
2021, e103811 (2021),
Abstract;
Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy: M. Schuldt, et al.; Front. Cardiovasc. Med.
8, 612215 (2021),
Abstract;
The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling: L. Pedrazza, et al.; Sci. Rep.
10, 824 (2020),
Abstract;
Full Text
Heat shock proteins in the physiology and pathophysiology of epidermal keratinocytes: D. Scieglinska, et al.; Cell Stress Chaperones
24, 1027 (2019),
Abstract;
Full Text
Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies: M. Haider, et al.; Autophagy
23, 1 (2019),
Abstract;
Establishing an Organotypic System for Investigating Multimodal Neural Repair Effects of Human Mesenchymal Stromal Stem Cells: D.K. Thakor, et al.; Curr. Protoc. Stem Cell Biol.
47, e58 (2018),
Abstract;
HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis : M. Sevin, et al.; Nat. Commun.
9, 1431 (2018),
Application(s): Immunoprecipitation,
Abstract;
Full Text
Use of protein-based biomarkers of exfoliated cervical cells for primary screening of cervical cancer: Y. Jin, et al.; Arch. Pharm. Res.
41, 438 (2018),
Abstract;
An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function: D. Mateju, et al.; EMBO J.
36, 1669 (2017),
Abstract;
Full Text
Conservative Mechanisms of Extracellular Trap Formation by Annelida Eisenia andrei: Serine Protease Activity Requirement: J. Homa, et al.; PLoS One
11, e0159031 (2016),
Application(s): Immunostaining,
Abstract;
Full Text
Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins: S. Karvinen, et al.; Exp. Gerontol.
79, 46 (2016),
Application(s): Immunoblotting,
Abstract;
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) Protein-Protein Interaction Inhibitor Reveals a Non-Catalytic Role for GAPDH Oligomerization in Cell Death: N. Qvit, et al.; J. Biol. Chem.
291, 13608 (2016),
Application(s): SDS-PAGE,
Abstract;
Full Text
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death: N. Qvit, et al.; J. Biol. Chem.
291, 13608 (2016),
Abstract;
Full Text
IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis: Y. Asano, et al.; Sci. Rep.
12, 19174 (2016),
Application(s): Western blot,
Abstract;
Full Text
Overcoming HSP27-mediated resistance by altered dimerization of HSP27 using small molecules: J.H. Kim, et al.; Oncotarget
7, 53178 (2016),
Abstract;
Full Text
Selective Phosphorylation Inhibitor of Delta Protein Kinase C-Pyruvate Dehydrogenase Kinase Protein-Protein Interactions: Application for Myocardial Injury in Vivo: N. Qvit, et al.; J. Am. Chem. Soc.
138, 7626 (2016),
Abstract;
Full Text
Twelve hours of heat stress induces inflammatory signaling in porcine skeletal muscle: S. Ganesan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
310, R1288 (2016),
Abstract;
Full Text
Genomic and proteomic investigation of preeclampsia: H. Sahin, et al.; Exp. Ther. Med.
10, 711 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Glutathione Levels and Susceptibility to Chemically Induced Injury in Two Human Prostate Cancer Cell Lines: L.H. Lash, et al.; Molecules
20, 10399 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation: V.T. Dang, et al.; PLoS One
9, e110748 (2014),
Abstract;
Full Text
HSF1 deficiency and impaired HSP90-dependent protein folding are hallmarks of aneuploid human cells: N. Donnelly, et al.; EMBO J.
33, 2374 (2014),
Abstract;
Full Text
OLA1 protects cells in heat shock by stabilizing HSP70: R.F. Mao, et al.; Cell Death Dis.
14, e491 (2013),
Abstract;
Full Text
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell
134, 769 (2008),
Application(s): WB using human cell lysates,
Abstract;
Cytotoxicity effects induced by Zearalenone metabolites, αZearalenol and β Zearalenol, on cultured Vero cells: Z. Ouanes-Ben Othmen, et al. ; Toxicology
252, 72 (2008),
Application(s): WB using monkey cell lysates,
Abstract;
HSP27 is involved in the pathogenesis of kidney tubulointerstitialfibrosis: A. Djamali, et al. ; Am. J. Physiol. Renal Physiol.
295, F707 (2008),
Application(s): WB using rat tissue,
Abstract;
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans: G. Paulsen, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
293, R844 (2007),
Application(s): ELISA, WB using human vastus lateralis muscle tissue,
Abstract;
Full Text
Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability: A.D. Verin, et al. ; FASEB J.
16, 1064 (2002),
Application(s): IP using bovine samples,
Abstract;
Estrogenic activity of procymidone in primary cultured rainbow trout hepatocytes (Oncorhynchus mykiss): E. Chiesara, et al. ; Toxicol. In Vitro
16, 475 (2002),
Application(s): WB using fish (trout) samples,
Abstract;
Modulation of heat-shock protein 27 (Hsp27) anti-apoptotic activity by methylglyoxal modification: T. Tsuruo, et al. ; J. Biol. Chem.
277, 45770 (2002),
Application(s): IP using human samples,
Abstract;
Subcorneal colocalization of the small heat shock protein, hsp27, with keratins and proteins of the cornified cell envelope: F. Trautinger, et al. ; Br. J. Dermatol.
147, 13 (2002),
Application(s): IHC using human samples,
Abstract;
Effect of the serine protease inhibitor N-tosyl-L-phenylalanine-chloromethyl ketone (TPCK) on MCF-7 mammary tumour cells growth and differentiation: P. Galand, et al. ; Arch. Biochem. Biophys.
24, 153 (2000),
Application(s): WB using mouse samples,
Abstract;
Differential expression of B-crystallin and Hsp27 in skeletal muscle during continuous contractile activity. Relationship to myogenic regulatory factors: I.J. Benjamin, et al. ; J. Biol. Chem.
271, 24089 (1996),
Application(s): WB using rabbit samples,
Abstract;
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