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Hip polyclonal antibody

ADI-SPA-766-D 50 µg 171.00 USD
ADI-SPA-766-F 200 µg 363.00 USD
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Product Specification

Alternative Name:HSC70-interacting protein
Immunogen:Recombinant rat Hip.
UniProt ID:P50503
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Applications:IHC, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~58kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Hip (Hsc70-interacting protein) is a cytosolic protein that exists as a homo-oligomer and participates in the regulation of Hsc70 in eukaryotic cells. Hip interacts with the ATPase domain of Hsc70 molecules through its tetratricopeptide repeats and flanking charged alpha-helices. Upon binding to Hsc70, Hip stabilizes the ADP-bound state of Hsc70, a conformation that has a high affinity for unfolded substrate proteins. Together with Hsp40 and BAG-1, Hip may regulate the chaperone activity of Hsc70 by stabilizing the chaperone-substrate complex.
Technical Info/Product Notes:Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000100380).
Regulatory Status:RUO - Research Use Only
Hip polyclonal antibody Western blot
Western blot analysis: Lane 1: MW marker, Lane 2: recombinant rat Hip, Lane 3: A431 cell lysate, Lane 4: mouse brain tissue extract, Lane 5: rat brain tissue extract, probed with Hip, pAb.
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Hip polyclonal antibody Western blot

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