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HSP70 (fish) polyclonal antibody

ADI-SPA-763-D 50 µl 269.00 USD
ADI-SPA-763-F 200 µl 487.00 USD
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Product Details

Alternative Name:Hsp70, HspA1A, Heat shock protein 70, HspA1B, Hsp72
Immunogen:Recombinant salmon Hsp70.
UniProt ID:Q91233
Source:Whole rabbit serum.
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Drosophila, Fish
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~70kDa by Western blot.
Purity Detail:Antiserum.
Formulation:Liquid. Whole rabbit serum.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Regulatory Status:RUO - Research Use Only
HSP70 (fish) polyclonal antibody Western blot
Western Blot Analysis of Hsp70 (fish), pAb (Prod. No. ADI-SPA-763): Lane 1: HSP70/HSP72 (Chinook Salmon) (recombinant) (Prod. No. ADI-SPP-763), Lane 2: Dnak (E.Coli) (recombinant) (Prod. No. ADI-SPP-630), Lane 3: RTG-2 Cell Lysate, Lane 4: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100), Lane 5: L-929 Cell Lysate, Lane 6: Rat-2 Cell Lysate, Lane 7: MDBK Cell Lysate, Lane 8: MDCK Cell Lysate, Lane 9: Drosophila Cell Lysate, Lane 10: Chicken Heart Lysate
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HSP70 (fish) polyclonal antibody Western blot

Product Literature References

Silver Nanoparticles Induce Degradation of the Endoplasmic Reticulum Stress Sensor Activating Transcription Factor-6 Leading to Activation of the NLRP-3 Inflammasome: J.C. Simard, et al.; J. Biol. Chem. 290, 5926 (2015), Application(s): Western Blotting, Abstract; Full Text
Effects of the Thermal Environment on Articular Chondrocyte Metabolism: A Fundamental Study to Facilitate Establishment of an Effective Thermotherapy for Osteoarthritis: A. Ito, et al.; J. Jpn. Phys. Ther. Assoc. 17, 14 (2014), Application(s): Western Blot, Abstract; Full Text

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