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ERp57 monoclonal antibody (MaP.Erp57)

ADI-SPA-725-D 50 µg 226.00 USD
ADI-SPA-725-F 200 µg 466.00 USD
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Product Details

Alternative Name:PDIA3, ERp60, ERp61, GRP57, Grp58
Immunogen:Recombinant human ERp57.
UniProt ID:P30101
GenBank ID:U42068
Source:Purified from ascites.
Species reactivity:Human
Dog, Hamster, Monkey, Porcine
Applications:IHC, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~58kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:ERp57 (also known as Grp58), a member of the Thioredoxin superfamily, is an ER protein with disulfide isomerase activity. It has been shown to increase after oncogenic transformation and to be a component of the MHC Class I peptide-loading complex.
Regulatory Status:RUO - Research Use Only
ERp57 monoclonal antibody (MaP.Erp57) Western blot
Western blot analysis of ERp57, mAb (MaP.Erp57) (Prod. no. ADI-SPA-725) : Lane 1: HeLa Cell Lysate, Lane 2: HeLa Cell Lysate (Heat Shocked), Lane 3: MDCK, Lane 4: MDCK (Heat Shocked), Lane 5: CHO-K1, Lane 6: ESK-4, Lane 7: Vero.
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ERp57 monoclonal antibody (MaP.Erp57) Western blot

Product Literature References

Early-onset torsion dystonia: a novel high-throughput yeast genetic screen for factors modifying protein levels of torsinAΔE: L.F. Zacchi, et al.; Dis. Model. Mech. 10, 1129 (2017), Abstract; Full Text
Effect of doxycycline-regulated ERp57 expression on specific thrombopoietin productivity of recombinant CHO cells: G.M. Lee, et al. ; Biotechnol. Prog. 19, 179 (2003), Application(s): WB using human samples, Abstract;
Proteomic analysis of lung adenocarcinoma: identification of a highly expressed set of proteins in tumors: D.G. Beer, et al. ; Clin. Cancer Res. 8, 2298 (2002), Application(s): IHC, WB using human samples, Abstract;
Recruitment of MHC class I molecules by tapasin into the transporter associated with antigen processing-associated complex is essential for optimal peptide loading: F. Momburg, et al. ; J. Immunol. 168, 1950 (2002), Application(s): WB using human samples, Abstract;

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