Product Details
| Alternative Name: | CALR, CRT |
| |
| Host: | Rabbit |
| |
| Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of human Calreticulin. |
| |
| UniProt ID: | P27797 |
| |
| GenBank ID: | AD000092 |
| |
| Source: | Purified from rabbit serum. |
| |
| Species reactivity: | Human, Mouse, Rat
Bovine, Dog
|
| |
| Applications: | ICC, IHC, IP, WB
|
| |
| Recommended Dilutions/Conditions: | Western Blot (1:1,000, human; 1:500, mouse; ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
| |
| Application Notes: | Predicted MW of ~63kDa. |
| |
| Purity Detail: | Protein A affinity purified. |
| |
| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | Calreticulin (CRT) is a multifunctional, multi-compartmental protein most abundant in the ER lumen. CRT contains the ER-retrieval sequence, KDEL, and has been best characterized as a soluble molecular chaperone of new or misfolded proteins and a Ca2+- binding protein. Both CRT and its membrane bound homolog, Calnexin (CNX) interact with proteins and glycoproteins that have monoglucosylated N-glycans. The CRT/CNX cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligimerization, regulates ER degradation, and provides quality control by preventing incompletely folded glycoproteins from exiting to the Golgi complex. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Western Blot Analysis of Calreticulin: Lane 1: MWM, Lane 2: HeLa cell lysate, Lane 3: PC-12 cell lysate. Lane 4: 3T3 cell lysate
Please mouse over
Product Literature References
RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy: J. Zhang, et al.; Nat. Commun.
13, 6007 (2022),
Abstract;
EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD: G. George, et al.; Elife
9, e53455 (2020),
Abstract;
Full Text
Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation: M. Shirane, et al.; Nat. Commun.
11, 4576 (2020),
Abstract;
Full Text
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods
15, 909 (2018),
Abstract;
Calreticulin Translocation Aggravates Endoplasmic Reticulum Stress-associated Apoptosis during Cardiomyocyte Hypoxia/Reoxygenation: F.F. Xu, et al.; Chin. Med. J. (Engl).
128, 353 (2015),
Application(s): Immunofluorescent Staining, Western Blotting,
Abstract;
Full Text
Cytosolically expressed PrP GPI-signal peptide interacts with mitochondria: G. Guizzunti & C. Zurzolo; Comm. Integr. Biol.
8, e1036206 (2015),
Abstract;
Full Text
NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein: M. Fausther, et al.; PLoS One
9, e98568 (2014),
Application(s): WB using monkey cell lysates,
Abstract;
Full Text
Distinct roles of mitochondria- and ER-localized Bcl-xL in apoptosisresistance and Ca2+ homeostasis: C.O. Eno, et al.; Mol. Biol. Cell
23, 2605 (2012),
Abstract;
Full Text
Dominant pro-vasopressin mutants that cause diabetes insipidus form disulfide-linked fibrillar aggregates in the endoplasmic reticulum: J. Birk, et al.; J. Cell Sci.
122, 3994 (2009),
Abstract;
Full Text
Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor: J. Lieberman, et al. ; Cell
112, 659 (2003),
Application(s): ICC, WB using human samples,
Abstract;
A-kinase anchor protein 84/121 are targeted to mitochondria and mitotic spindles by overlapping amino-terminal motifs: A. Feliciello, et al. ; J. Mol. Biol.
320, 663 (2002),
Application(s): ICC using mouse samples,
Abstract;
Bcl-Xl affects Ca2+ homeostasis by altering expression of inositol 1,4,5-trisphosphate receptors: C.B. Thompson, et al. ; PNAS
99, 9830 (2002),
Application(s): WB using mouse samples,
Abstract;
Myelin proteolipid protein forms a complex with integrins and may participate in integrin receptor signaling in oligodendrocytes: W.B. Macklin, et al. ; J. Neurosci.
22, 7398 (2002),
Application(s): ICC, WB using rat samples,
Abstract;
Pseudoachondroplasia is caused through both intra- and extracellular pathogenic pathways: P. Maurer, et al. ; J. Clin. Invest.
110, 505 (2002),
Application(s): IP using bovine samples,
Abstract;
The anti-adhesive activity of thrombospondin is mediated by the N-terminal domain of cell surface calreticulin: J.E. Murphy-Ullrich, et al. ; J. Biol. Chem.
277, 37219 (2002),
Application(s): ICC using bovine samples,
Abstract;
Folding of hepatitis C virus E1 glycoprotein in a cell-free system: S. Abrignani, et al. ; J. Virol.
75, 11205 (2001),
Application(s): IP using canine samples,
Abstract;
Calnexin and calreticulin binding to human thyroperoxidase is required for its first folding step(s) but is not sufficient to promote efficient cell surface expression: J.L. Franc, et al. ; Endocrinology
141, 959 (2000),
Application(s): IP using human samples,
Abstract;
Genetic evidence for selective transport of opsin and arrestin by kinesin-II in mammalian photoreceptors: S.B. Goldstein, et al. ; Cell
102, 175 (2000),
Application(s): IHC using mouse samples,
Abstract;
ERcalcistorin/protein-disulfide isomerase acts as a calcium storage protein in the endoplasmic reticulum of a living cell. Comparison with calreticulin and calsequestrin: B. Kaminer, et al. ; J. Biol. Chem.
273, 9857 (1998),
Application(s): ICC, WB using human samples,
Abstract;
Related Products
