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Metallothionein monoclonal antibody (UC1MT)

ADI-SPA-550-D 50 µg 256.00 USD
ADI-SPA-550-F 200 µg 510.00 USD
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Product Details

Alternative Name:MT1A, Metallothionein MGC32848, MT1S, MTC, metallothionein 1A (functional)
Immunogen:Cross-linked rabbit liver Metallothionein I and II.
Source:Purified from ascites.
Species reactivity:Human, Mouse, Rat
Fish, Horse, Rabbit
Applications:ELISA, IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~15kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.
Regulatory Status:RUO - Research Use Only
Metallothionein monoclonal antibody (UC1MT) Western blot
Western blot analysis of Prod. No. ADI-SPA-550: Lane 1: MW marker, Lane 2: MT I, Lane 3: MT II, Lane 4: Mummichug CdCl2.
Metallothionein monoclonal antibody (UC1MT) Immunohistochemistry
Immunohistochemistry analysis of human uterus tissue stained with Metallothionein, mAb (UC1MT) at 10µg/ml.
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Metallothionein monoclonal antibody (UC1MT) Western blot Metallothionein monoclonal antibody (UC1MT) Immunohistochemistry

Product Literature References

The protease DDI2 regulates NRF1 activation in response to cadmium toxicity: S.T. Ribeiro, et al.; iScience 25, 105227 (2022), Abstract;
Morphofunctional Alterations in Zebrafish (Danio rerio) Gills after Exposure to Mercury Chloride.: R. Macirella & E. Brunelli; Int. J. Mol. Sci. 18, 824 (2017), Abstract; Full Text
Effects of Two Sublethal Concentrations of Mercury Chloride on the Morphology and Metallothionein Activity in the Liver of Zebrafish (Danio rerio): R. Macirella, et al.; Int. J. Mol. Sci. 17, e361 (2016), Application(s): Immunohistochemistry, Abstract; Full Text
Metallothioneins and heat shock proteins 70 in Armadillidium vulgare (Isopoda, Oniscidea) exposed to cadmium and lead: V. Mazzei, et al.; Ecotoxicol. Environ. Saf. 116, 99 (2015), Application(s): Immunohistochemistry, Western Blotting , Abstract;
Identification and spatial analysis of metallothioneins expressed by the adult human lens: M. Kantorow, et al. ; Invest. Ophthalmol. Vis. Sci. 42, 188 (2001), Application(s): WB using human samples, Abstract;
Potentiation of cadmium-induced cytotoxicity by sulfur amino acid deprivation through activation of extracellular signal-regulated kinase1/2 (ERK1/2) in conjunction with p38 kinase or c-jun N-terminal kinase (JNK). Complete inhibition of the potentiated t: S.G. Kim, et al. ; Biochem. Pharmacol. 62, 1379 (2001), Application(s): WB using rat samples, Abstract;
Immunomodulatory activities of extracellular metallothionein. I. Metallothionein effects on antibody production: E.A. Olson, et al. ; Toxicology 85, 161 (1993), Application(s): EIA (RB), WB using horse & rabbit samples, Abstract;

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