[pSer⁷⁸]HSP27 polyclonal antibody

[pSer78]HSP27 polyclonal antibody

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ADI-SPA-523-D	50 µg	202.00 USD

ADI-SPA-523-F	200 µg	457.00 USD
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Replaces Prod. #: BML-SA442

Alternative Name:	HspB1, Heat shock protein 27

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to a portion of human HSP27 phosphorylated at Ser⁷⁸.

UniProt ID:	P04792

GenBank ID:	L39370

Source:	Purified from rabbit serum.

Species reactivity:	Human, Rat Bovine, Guinea pig, Monkey

Applications:	IHC, WB

Recommended Dilutions/Conditions:	Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~27kDa by Western blot.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Regulatory Status:	RUO - Research Use Only

[pSer78]HSP27 polyclonal antibody Western blot

Western blot analysis of HSP27 (pSer78), pAb: Lane 1 MW marker, Lane 2 phospho HSP27 (Prod. No. ADI-SPP-716), Lane 3 non-phospho HSP27 (Prod. No. ADI-SPP-715) and Lane 4 MDBK (weak reactivity).

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HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress: J.S. Gaston, et al.; Cell. Mol. Life Sci. 79, 548 (2022), Abstract;

Histone deacetylase inhibitor SAHA treatment prevents the development of heart failure after myocardial infarction via an induction of heat-shock proteins in rats: S. Nagata, et al.; Biol. Pharm. Bull. 42, 453 (2019), Abstract;

Release of Phosphorylated HSP27 (HSPB1) from Platelets Is Accompanied with the Acceleration of Aggregation in Diabetic Patients: H. Tokuda, et al.; PLoS One 10, e0128977 (2015), Application(s): Western Blot, Abstract; Full Text

Different glial response to methamphetamine- and methylenedioxymethamphetamine-induced neurotoxicity: E. Escubedo, et al. ; Naunyn Schmiedebergs Arch. Pharmacol. 367, 490 (2003), Application(s): IHC using rat samples, Abstract;

Datasheet
SDS
Certificate of Analysis

By target:

HSP20

By biological activity:

HSP20 Polyclonal antibody

By catalog section:

Small Heat Shock Proteins

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Goat anti-rabbit IgG, polyclonal antibody (HRP conjugate)

ELISA, IHC, WB | Print as PDF

ADI-SAB-300-J

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