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HSP47 monoclonal antibody (M16.10A1)

ADI-SPA-470-D 50 µg 210.00 USD
ADI-SPA-470-F 200 µg 421.00 USD
ADI-SPA-470-J 1 mg 1,752.00 USD
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Product Specification

Alternative Name:SerpinH1, Colligin, Gp46, Heat shock protein 47, Collagen-binding protein
Immunogen:Native rat HSP47.
UniProt ID:P50454
GenBank ID:X61598
Source:Purified from ascites.
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
Applications:IHC, WB
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~47kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion.
Regulatory Status:RUO - Research Use Only
HSP47 monoclonal antibody (M16.10A1) Western blot
Western blot analysis of HSP47, mAb (M16.10A1) (Prod. no. ADI-SPA-470): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).
HSP47 monoclonal antibody (M16.10A1) Immunohistochemistry
Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).
HSP47 monoclonal antibody (M16.10A1) Immunohistochemistry
Immunohistochemistry analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).
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HSP47 monoclonal antibody (M16.10A1) Western blot HSP47 monoclonal antibody (M16.10A1) Immunohistochemistry HSP47 monoclonal antibody (M16.10A1) Immunohistochemistry

Product Literature References

HSP47 promotes metastasis of breast cancer by interacting with myosin IIA via the unfolded protein response transducer IRE1α: A. Yoneda, et al.; Oncogene 39, 4519 (2020), Application(s): Flow cytometry and immunofluorescence using breast cancer cell lines, Abstract;
The inducible amphisome isolates viral hemagglutinin and defends against influenza A virus infection: J. Omi, et al.; Nat. Commun. 11, 162 (2020), Application(s): Immunofluorescence using MDCK cells, Abstract; Full Text
Mass spectrometry-based proteomics to define intracellular collagen interactomes: N.D. Doan, et al.; Methods Mol. Biol. 1944, 95 (2019), Application(s): Western-blot, Abstract; Full Text
Interactome Screening Identifies the ER Luminal Chaperone Hsp47 as a Regulator of the Unfolded Protein Response Transducer IRE1α: D. Sepulveda, et al.; Mol. Cell 69, 238 (2018), Abstract;
Severe disruption and disorganization of dermal collagen fibrils in early striae gravidarum: F. Wang, et al.; Br. J. Dermatol. 178, 749 (2018), Abstract;
Therapeutic potential of tranilast for the treatment of chronic graft-versus-host disease in mice: S. Mukai, et al.; PLoS One 13, (2018), Abstract; Full Text
Cell-free Generation of COPII-coated Procollagen I Carriers: L. Yuan, et al.; Bio Protoc. 7, e2450 (2017), Abstract; Full Text
Defective signaling, osteoblastogenesis and bone remodeling in a mouse model of connexin 43 C-terminal truncation: M.C. Moorer, et al.; J. Cell Sci. 130, 531 (2017), Abstract; Full Text
Heat shock protein 47 and 65-kDa FK506-binding protein weakly but synergistically interact during collagen folding in the endoplasmic reticulum: Y. Ishikawa, et al.; J. Biol. Chem. 292, 17216 (2017), Abstract; Full Text
Proteomics analysis of differential protein expression identifies Heat shock protein 47 as a predictive marker for lymph node metastasis in patients with colorectal cancer: K. Mori, et al.; Int. J. Cancer 140, 1425 (2017), Abstract;
Immunopathologic characterization of ultrasound-defined synovitis in rheumatoid arthritis patients in clinical remission: J. Ramirez, et al.; Arthritis Res. Ther. 18, 74 (2016), Application(s): For synovial fibroblasts, Abstract; Full Text
Mutants of collagen-specific molecular chaperone Hsp47 causing osteogenesis imperfecta are structurally unstable with weak binding affinity to collagen: S. Ito, et al; Biochem. Biophys. Res. Commun. 469, 437 (2016), Application(s): Western blot, Abstract;
Wound healing profiles of hyperopic-small incision lenticule extraction (SMILE): Y.C. Liu, et al.; Sci. Rep. 6, 29802 (2016), Application(s): Histology and immunohistochemistry, rabbit cornea, Abstract; Full Text
Molecular Consequences of Defective SERPINH1/HSP47 in the Dachshund Natural Model of Osteogenesis Imperfecta: U. Lindert, et al.; J. Biol. Chem. 290, 17679 (2015), Application(s): Immunofluorescence, Western Blot, Abstract; Full Text
The Kampo Medicine Daikenchuto Inhibits Peritoneal Fibrosis in Mice: M. Kitamura, et al.; Biol. Pharm. Bull. 38, 193 (2015), Application(s): Western Blotting and Immunohistochemistry, Abstract; Full Text
Yip1A, a Novel Host Factor for the Activation of the IRE1 Pathway of the Unfolded Protein Response during Brucella Infection: Y. Taguchi, et al.; PLoS Pathog. 11, e1004747 (2015), Application(s): Immunoprecipitation, Abstract; Full Text
Bleomycin delivery by osmotic minipump: similarity to human scleroderma interstitial lung disease: R. Lee, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 306, L736 (2014), Abstract;
Primary fibroblasts of NDUFS4-/-mice display increased ROS levels and aberrant mitochondrial morphology: F. Valsecchi, et al.; Mitochondrion 13, 436 (2013), Application(s): Immunocytochemistry using primary murine muscle cells, Abstract;
Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: The roles of Hsp47 and basic fibroblast growth factor: C.J. Xu, et al.; Pathol. Res. Pract. 209, 69 (2013), Application(s): Immunohistochemistry using human formalin-fixed paraffin-embedded colorectal carcinoma tissue sections, Abstract;
Novel Adenoviral Gene Delivery System Targeted Against Head and NeckCancer: D. Li, et al. ; Laryngoscope 118, 650 (2008), Application(s): Flow, IF using human & hamster tissue culture, Abstract;
Renoprotective Effect of Azelnidipine in Rats: A. Furusu, et al. ; Biol. Pharm. Bull. 31, 2237 (2008), Application(s): IHC using rat samples, Abstract;
Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy: M.M. Smith, et al.; Rheumatology 47, 1172 (2008), Application(s): IHC using sheep tissue, Abstract; Full Text
Col4a1 mutation causes endoplasmic reticulum stress and genetically modifiable ocular dysgenesis: D.B. Gould, et al.; Hum. Mol. Genet. 16, 798 (2007), Application(s): IHC using mouse tissue, Abstract; Full Text
Role of heat shock protein 47, a collagen-binding chaperone, inlacrimal gland pathology in patients with cGVHD: Y. Ogawa, et al. ; Invest. Ophthalmol. Vis. Sci. 48, 1079 (2007), Application(s): IHC, IF, WB using human tissue & cell lysates, Abstract;
Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins: T. Koide, et al.; J. Biol. Chem. 281, 11177 (2006), Application(s): WB using recombinant GST-mouse HSP47 fusion protein, Abstract; Full Text
Specific recognition of the collagen triple helix by chaperone HSP47: minimal structural requirement and spatial molecular orientation.: T. Koide, et al.; J. Biol. Chem. 281, 3432 (2006), Application(s): WB using recombinant human and mouse HSP47, Abstract; Full Text
Proteomic Analysis of Primary Cultures of Human Adipose-derived Stem Cells: Modulation by Adipogenesis: J.P. DeLany, et al.; Mol. Cell. Proteomics 4, 731 (2005), Application(s): WB using human adipose tissue cell lysates, Abstract; Full Text
Immunohistochemical Characterization of Cells in Adult Human Patellar Tendons: F.S. Chuen, et al.; J. Histochem. Cytochem. 52, 1151 (2004), Application(s): IHC using adult human patellar tendons, Abstract; Full Text
Immunohistochemical Expression of Angiogenesis-Related Markers in Oral Squamous Cell Carcinomas With Multiple Metastatic Lymph Nodes: N.G. Nikitakis, et al.; Am. J. Clin. Pathol. 119, 574 (2003), Application(s): IHC using human oral squamous cell carcinomas (SCCs), Abstract; Full Text
Localisation and phenotypical characterisation of collagen-producing cells in TGF-beta1-induced renal interstitial fibrosis: L. Wogensen, et al. ; Histochem. Cell Biol. 119, 267 (2003), Application(s): IHC, WB using mouse samples, Abstract;
Prevalence of HSP47 antigen and autoantibodies to HSP47 in the sera of patients with mixed connective tissue disease: K. Nagata, et al. ; Biochem. Biophys. Res. Commun. 303, 413 (2003), Application(s): EIA using human samples, Abstract;
Functional Linkage between the Endoplasmic Reticulum Protein Hsp47 and Procollagen Expression in Human Vascular Smooth Muscle Cells: J.G. Pickering, et al. ; J. Biol. Chem. 277, 38571 (2002), Application(s): WB using human samples, Abstract;
Sequence-specific recognition of collagen triple helices by the collagen-specific molecular chaperone HSP47: N.J. Bulleid, et al. ; J. Biol. Chem. 277, 35007 (2002), Application(s): IP using human samples, Abstract;
HSP47 expression by smooth muscle cells is increased during arterial development and lesion formation and is inhibited by fibrillar collagen: J.G. Pickering, et al. ; Arterioscler. Thromb. Vasc. Biol. 21, 40 (2001), Application(s): WB using rat samples, Abstract;
Role of atrophic tubules in development of interstitial fibrosis in microembolism-induced renal failure in rat: A. Hishida, et al. ; Am. J. Pathol. 158, 75 (2001), Application(s): IHC using rat samples, Abstract;
Intracellular Retention of Procollagen within the Endoplasmic Reticulum Is Mediated by Prolyl 4-Hydroxylase: A.R. Walmsley, et al.; J. Biol. Chem. 274, 14884 (1999), Application(s): IP using human HT1080 cells, Abstract; Full Text

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