Product Details
| Alternative Name: | SerpinH1, Colligin, Gp46, Heat shock protein 47, Collagen-binding protein |
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| Clone: | M16.10A1 |
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| Host: | Mouse |
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| Isotype: | IgG2bλ |
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| Immunogen: | Native rat HSP47. |
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| UniProt ID: | P50454 |
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| GenBank ID: | X61598 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
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| Applications: | IHC, WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (1:50)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~47kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion. |
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| Technical Info/Product Notes: | Cited samples:
For an overview on cited samples please click here. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of HSP47, mAb (M16.10A1) (Prod. no. ADI-SPA-470): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).
Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).
Immunohistochemistry analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).
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Product Literature References
Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis: N.M. Farràs, et al.; Nucleic Acids Res.
50, 8207 (2022),
Abstract;
AAV-mediated YAP expression in cardiac fibroblasts promotes inflammation and increases fibrosis: J. Francisco, et al.; Sci. Rep.
11, 10553 (2021),
Abstract;
Full Text
Eyelid blood vessel and meibomian gland changes in a sclerodermatous chronic GVHD mouse model: F. Yang, et al.; Ocul. Surf. (2021),
Abstract;
Heat shock protein 47 confers chemoresistance on pancreatic cancer cells by interacting with calreticulin and IRE1α: A. Yoneda, et al.; Cancer Sci.
112, 2803 (2021),
Abstract;
Chronic heat stress regulates the relation between heat shock protein and immunity in broiler small intestine: S.H. Siddiqui, et al.; Sci. Rep.
10, 18872 (2020),
Abstract;
Full Text
HSP47 promotes metastasis of breast cancer by interacting with myosin IIA via the unfolded protein response transducer IRE1α: A. Yoneda, et al.; Oncogene
39, 4519 (2020),
Application(s): Flow cytometry and immunofluorescence using breast cancer cell lines,
Abstract;
The inducible amphisome isolates viral hemagglutinin and defends against influenza A virus infection: J. Omi, et al.; Nat. Commun.
11, 162 (2020),
Application(s): Immunofluorescence using MDCK cells,
Abstract;
Full Text
Mass spectrometry-based proteomics to define intracellular collagen interactomes: N.D. Doan, et al.; Methods Mol. Biol.
1944, 95 (2019),
Application(s): Western-blot,
Abstract;
Full Text
Interactome Screening Identifies the ER Luminal Chaperone Hsp47 as a Regulator of the Unfolded Protein Response Transducer IRE1α: D. Sepulveda, et al.; Mol. Cell
69, 238 (2018),
Abstract;
Severe disruption and disorganization of dermal collagen fibrils in early striae gravidarum: F. Wang, et al.; Br. J. Dermatol.
178, 749 (2018),
Abstract;
Therapeutic potential of tranilast for the treatment of chronic graft-versus-host disease in mice: S. Mukai, et al.; PLoS One
13, e0203742 (2018),
Abstract;
Full Text
Cell-free Generation of COPII-coated Procollagen I Carriers: L. Yuan, et al.; Bio Protoc.
7, e2450 (2017),
Abstract;
Full Text
Defective signaling, osteoblastogenesis and bone remodeling in a mouse model of connexin 43 C-terminal truncation: M.C. Moorer, et al.; J. Cell Sci.
130, 531 (2017),
Abstract;
Full Text
Heat shock protein 47 and 65-kDa FK506-binding protein weakly but synergistically interact during collagen folding in the endoplasmic reticulum: Y. Ishikawa, et al.; J. Biol. Chem.
292, 17216 (2017),
Abstract;
Full Text
Proteomics analysis of differential protein expression identifies Heat shock protein 47 as a predictive marker for lymph node metastasis in patients with colorectal cancer: K. Mori, et al.; Int. J. Cancer
140, 1425 (2017),
Abstract;
Immunopathologic characterization of ultrasound-defined synovitis in rheumatoid arthritis patients in clinical remission: J. Ramirez, et al.; Arthritis Res. Ther.
18, 74 (2016),
Application(s): For synovial fibroblasts,
Abstract;
Full Text
Mutants of collagen-specific molecular chaperone Hsp47 causing osteogenesis imperfecta are structurally unstable with weak binding affinity to collagen: S. Ito, et al; Biochem. Biophys. Res. Commun.
469, 437 (2016),
Application(s): Western blot,
Abstract;
Wound healing profiles of hyperopic-small incision lenticule extraction (SMILE): Y.C. Liu, et al.; Sci. Rep.
6, 29802 (2016),
Application(s): Histology and immunohistochemistry, rabbit cornea,
Abstract;
Full Text
Molecular Consequences of Defective SERPINH1/HSP47 in the Dachshund Natural Model of Osteogenesis Imperfecta: U. Lindert, et al.; J. Biol. Chem.
290, 17679 (2015),
Application(s): Immunofluorescence, Western Blot,
Abstract;
Full Text
The Kampo Medicine Daikenchuto Inhibits Peritoneal Fibrosis in Mice: M. Kitamura, et al.; Biol. Pharm. Bull.
38, 193 (2015),
Application(s): Western Blotting and Immunohistochemistry,
Abstract;
Full Text
Yip1A, a Novel Host Factor for the Activation of the IRE1 Pathway of the Unfolded Protein Response during Brucella Infection: Y. Taguchi, et al.; PLoS Pathog.
11, e1004747 (2015),
Application(s): Immunoprecipitation,
Abstract;
Full Text
Bleomycin delivery by osmotic minipump: similarity to human scleroderma interstitial lung disease: R. Lee, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol.
306, L736 (2014),
Abstract;
Primary fibroblasts of NDUFS4-/-mice display increased ROS levels and aberrant mitochondrial morphology: F. Valsecchi, et al.; Mitochondrion
13, 436 (2013),
Application(s): Immunocytochemistry using primary murine muscle cells,
Abstract;
Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: The roles of Hsp47 and basic fibroblast growth factor: C.J. Xu, et al.; Pathol. Res. Pract.
209, 69 (2013),
Application(s): Immunohistochemistry using human formalin-fixed paraffin-embedded colorectal carcinoma tissue sections,
Abstract;
Novel Adenoviral Gene Delivery System Targeted Against Head and NeckCancer: D. Li, et al. ; Laryngoscope
118, 650 (2008),
Application(s): Flow, IF using human & hamster tissue culture,
Abstract;
Renoprotective Effect of Azelnidipine in Rats: A. Furusu, et al. ; Biol. Pharm. Bull.
31, 2237 (2008),
Application(s): IHC using rat samples,
Abstract;
Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy: M.M. Smith, et al.; Rheumatology
47, 1172 (2008),
Application(s): IHC using sheep tissue,
Abstract;
Full Text
Col4a1 mutation causes endoplasmic reticulum stress and genetically modifiable ocular dysgenesis: D.B. Gould, et al.; Hum. Mol. Genet.
16, 798 (2007),
Application(s): IHC using mouse tissue,
Abstract;
Full Text
Role of heat shock protein 47, a collagen-binding chaperone, inlacrimal gland pathology in patients with cGVHD: Y. Ogawa, et al. ; Invest. Ophthalmol. Vis. Sci.
48, 1079 (2007),
Application(s): IHC, IF, WB using human tissue & cell lysates,
Abstract;
Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins: T. Koide, et al.; J. Biol. Chem.
281, 11177 (2006),
Application(s): WB using recombinant GST-mouse HSP47 fusion protein,
Abstract;
Full Text
Specific recognition of the collagen triple helix by chaperone HSP47: minimal structural requirement and spatial molecular orientation.: T. Koide, et al.; J. Biol. Chem.
281, 3432 (2006),
Application(s): WB using recombinant human and mouse HSP47,
Abstract;
Full Text
Proteomic Analysis of Primary Cultures of Human Adipose-derived Stem Cells: Modulation by Adipogenesis: J.P. DeLany, et al.; Mol. Cell. Proteomics
4, 731 (2005),
Application(s): WB using human adipose tissue cell lysates,
Abstract;
Full Text
Immunohistochemical Characterization of Cells in Adult Human Patellar Tendons: F.S. Chuen, et al.; J. Histochem. Cytochem.
52, 1151 (2004),
Application(s): IHC using adult human patellar tendons,
Abstract;
Full Text
Immunohistochemical Expression of Angiogenesis-Related Markers in Oral Squamous Cell Carcinomas With Multiple Metastatic Lymph Nodes: N.G. Nikitakis, et al.; Am. J. Clin. Pathol.
119, 574 (2003),
Application(s): IHC using human oral squamous cell carcinomas (SCCs),
Abstract;
Full Text
Localisation and phenotypical characterisation of collagen-producing cells in TGF-beta1-induced renal interstitial fibrosis: L. Wogensen, et al. ; Histochem. Cell Biol.
119, 267 (2003),
Application(s): IHC, WB using mouse samples,
Abstract;
Prevalence of HSP47 antigen and autoantibodies to HSP47 in the sera of patients with mixed connective tissue disease: K. Nagata, et al. ; Biochem. Biophys. Res. Commun.
303, 413 (2003),
Application(s): EIA using human samples,
Abstract;
Functional Linkage between the Endoplasmic Reticulum Protein Hsp47 and Procollagen Expression in Human Vascular Smooth Muscle Cells: J.G. Pickering, et al. ; J. Biol. Chem.
277, 38571 (2002),
Application(s): WB using human samples,
Abstract;
Sequence-specific recognition of collagen triple helices by the collagen-specific molecular chaperone HSP47: N.J. Bulleid, et al. ; J. Biol. Chem.
277, 35007 (2002),
Application(s): IP using human samples,
Abstract;
HSP47 expression by smooth muscle cells is increased during arterial development and lesion formation and is inhibited by fibrillar collagen: J.G. Pickering, et al. ; Arterioscler. Thromb. Vasc. Biol.
21, 40 (2001),
Application(s): WB using rat samples,
Abstract;
Role of atrophic tubules in development of interstitial fibrosis in microembolism-induced renal failure in rat: A. Hishida, et al. ; Am. J. Pathol.
158, 75 (2001),
Application(s): IHC using rat samples,
Abstract;
Intracellular Retention of Procollagen within the Endoplasmic Reticulum Is Mediated by Prolyl 4-Hydroxylase: A.R. Walmsley, et al.; J. Biol. Chem.
274, 14884 (1999),
Application(s): IP using human HT1080 cells,
Abstract;
Full Text
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