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HSP40/Hdj1 monoclonal antibody (2E1)

ADI-SPA-450-E 100 µg 405.00 USD
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Product Details

Alternative Name:Heat shock protein 40
Immunogen:Recombinant human Hsp40 (Hdj1).
UniProt ID:P25685
GenBank ID:D49547
Species reactivity:Human, Mouse, Rat
Applications:IP, WB
Recommended Dilutions/Conditions:Immunoprecipitation (2-5µg/200µl from 5x106cells)
Western Blot (1-2µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~40kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Hsp40/Hdj1 is a cytosolic co-chaperone belonging to the class DnaJ, named after its homolog in E. coli. Hsp40 functions in protein folding by binding nascent peptides and unfolded substrates and facilitating substrate interaction with Hsp70. Binding of Hsp40 to Hsp70 increases Hsp70 ATP hydrolysis and substrate binding, which is reversed by the action of nucleotide exchange factors (GrpE in E. coli). Repeated cycles of Hsp40/Hsp70 peptide binding and ATP hydrolysis prevent premature folding and aggregation, promoting protein maturation throughout the cell.
Regulatory Status:RUO - Research Use Only
HSP40/Hdj1 monoclonal antibody (2E1) Western blot
Western blot analysis of Jurkat (1), Raji (2), HeLa (3), MRC-5 (4), ZR-75-1 (5), NIH/3T3 (6), WR19L (7), P19 (8) and PC12 (9) using HSP40 (Hdj1), mAb (2E1).
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HSP40/Hdj1 monoclonal antibody (2E1) Western blot

Product Literature References

Protein quality control in the nucleolus safeguards recovery of epigenetic regulators after heat shock: M. Azkanaz, et al.; Elife 8, e45205 (2019), Abstract; Full Text
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients: M. Wattin, et al.; Int. J. Mol. Sci. 19, 178 (2018), Application(s): Western Blot, Abstract; Full Text
Mitochondrial HSP70, HSP40, and HSP60 bind to the 3' untranslated region of the Murine hepatitis virus genome: J.L. Leibowitz, et al. ; Arch. Virol. 149, 93 (2004), Application(s): WB using mouse samples, Abstract;

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