Product Details
| Host: | Rabbit |
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| Immunogen: | Native bovine Ubiquitin. |
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| UniProt ID: | P0CG53 |
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| Gene/Protein Identifier: | NP_776558 (RefSeq) |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Chicken, Dog, Drosophila, Fish, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus, Yeast
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| Applications: | ICC, IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:5,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~8kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by the 26S complex, a multicatalytic cytosolic and nuclear protease. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human brain, cortex, senile plaque tissue stained with Ubiquitin, pAb at 10μg/ml.
Western blot analysis: Ubiquitin protein probed with Ubiquitin pAb.
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Product Literature References
MLN4924 Inhibits Defective Ribosomal Product Antigen Presentation Independently of Direct NEDDylation of Protein Antigens: K. Vijayasimha, et al.; J. Immunol.
208, 2273 (2022),
Abstract;
Disrupting the ghrelin-growth hormone axis limits ghrelin's orexigenic but not glucoregulatory actions: R.S. Eshaq, et al.; Microcirculation
28, e12717 (2021),
Abstract;
USP18 promotes tumor metastasis in esophageal squamous cell carcinomas via deubiquitinating ZEB1: C. Song, et al.; Exp. Cell Res.
409, 112884 (2021),
Abstract;
Trehalose alleviates the phenotype of Machado-Joseph disease mouse models: M.M. Santana, et al.; J. Transl. Med.
18, 161 (2020),
Abstract;
Full Text
Correcting errors in the BRCA1 warning system: Y. Liang, et al.; DNA Repair
73, 120 (2018),
Abstract;
Epidithiodiketopiperazines Inhibit Protein Degradation by Targeting Proteasome Deubiquitinase Rpn11: J. Li, et al.; Cell Chem. Biol.
25, 1350 (2018),
Abstract;
PCB-126 effects on aryl hydrocarbon receptor, ubiquitin and p53 expression levels in a fish product (Sparus aurata L.): M. Calo, et al.; Nat. Prod. Res.
32, 1136 (2018),
Abstract;
ARIH2 Ubiquitinates NLRP3 and Negatively Regulates NLRP3 Inflammasome Activation in Macrophages: A. Kawashima, et al.; J. Immunol.
199, 3614 (2017),
Abstract;
Astaxanthin intake attenuates muscle atrophy caused by immobilization in rats: T. Shibaguchi, et al.; Physiol. Rep.
4, e12885 (2016),
Application(s): SDS-PAGE and western blotting,
Abstract;
Full Text
TAX1BP1 Restrains Virus-Induced Apoptosis by Facilitating Itch-Mediated Degradation of the Mitochondrial Adaptor MAVS: Y.B. Choi, et al.; Mol. Cell. Biol.
37, e00422-16 (2016),
Abstract;
Full Text
Disruption of polyubiquitin gene Ubc leads to attenuated resistance against arsenite-induced toxicity in mouse embryonic fibroblasts: M.N. Kim, et al.; Biochim. Biophys. Acta
1853, 996 (2015),
Application(s): Western Blotting,
Abstract;
SCA7 knockin mice model human SCA7 and reveal gradual accumulation of mutant ataxin-7 in neurons and abnormalities in short-term plasticity: H.Y. Zoghbi, et al. ; Neuron
37, 383 (2003),
Application(s): ICC using mouse samples,
Abstract;
von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination: M.F. Czyzyk-Krzeska, et al. ; PNAS
100, 2706 (2003),
Application(s): WB using rat samples,
Abstract;
p62 is a common component of cytoplasmic inclusions in protein aggregation diseases: H. Denk, et al. ; Am. J. Pathol.
160, 255 (2002),
Application(s): IHC using mouse samples,
Abstract;
Phosphorylation of retinoid X receptor suppresses its ubiquitination in human hepatocellular carcinoma: Y. Okano, et al. ; Hepatology
35, 332 (2002),
Application(s): WB using human samples,
Abstract;
HIV protease inhibitors protect apolipoprotein B from degradation by the proteasome: a potential mechanism for protease inhibitor-induced hyperlipidemia: S.L. Sturley, et al. ; Nat. Med.
7, 1327 (2001),
Application(s): IP using human samples,
Abstract;
Impairment of bile salt-dependent lipase secretion in AR4-2J rat pancreatic cells induces its degradation by the proteasome: N. Bruneau, et al. ; Biochim. Biophys. Acta
1530, 184 (2001),
Application(s): WB using rat samples,
Abstract;
Activation-dependent degradation of protein kinase C eta: C.S. Hahn, et al. ; Oncogene
19, 4263 (2000),
Application(s): WB using human samples,
Abstract;
The polyubiquitin gene is essential for meiosis in fission yeast: O. Niwa, et al. ; Exp. Cell Res.
254, 143 (2000),
Application(s): WB using yeast samples,
Abstract;
Tumor necrosis factor-alpha inhibits expression of CTP: phosphocholine cytidylyl transferase: S. Jackowski, et al. ; J. Biol. Chem.
275, 9699 (2000),
Application(s): IP, WB using mouse samples,
Abstract;
Ubiquitination of the human immunodeficiency virus type 1 Env glycoprotein: J. Haas, et al. ; J. Virol.
74, 5373 (2000),
Application(s): IP using mouse samples,
Abstract;
The degradation of apolipoprotein B100 is mediated by the ubiquitin-proteasome pathway and involves heat shock protein 70: H.N. Ginsberg, et al. ; J. Biol. Chem.
272, 20427 (1997),
Application(s): IP using human samples,
Abstract;
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