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Mn SOD polyclonal antibody

ADI-SOD-111-D 50 µg 299.00 USD
ADI-SOD-111-F 200 µg 556.00 USD
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Product Details

Alternative Name:Manganese superoxide dismutase
Immunogen:Native rat Mn SOD.
UniProt ID:P07895
Gene/Protein Identifier:NP_058747 (RefSeq)
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Clam, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
Applications:ELISA, Flow Cytometry, IHC (PS), IP, WB
Recommended Dilutions/Conditions:Flow Cytometry (1:100)
Immunoprecipitation (1:100)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~25kDa by Western blot.
Purity Detail:Immunoaffinity purified.
Formulation:Liquid. In sodium phosphate buffer, pH 7.0, containing 15mg/ml BSA, 0.09% sodium azide, and 50% glycerol.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:SOD (Superoxide dismutase) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide. There are three SOD isoenzymes in mammalian cells, they are: EC SOD (extracellular SOD), Cu/Zn SOD (copper and zinc-containing SOD) and Mn SOD (manganese-containing SOD).
Regulatory Status:RUO - Research Use Only
Mn SOD polyclonal antibody Flow Cytometry
Flow cytometry analysis of U251 cells using Mn SOD, pAb (right) and control rabbit IgG (left).
Mn SOD polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis of human skeletal muscle tissue stained with Mn SOD, pAb at 10µg/ml.
Mn SOD polyclonal antibody Western blot
Western blot analysis of MnSOD pAb: Lane 1: HeLa Cell Lysate, Lane 2: Rat Brain Tissue Extract, Lane 3: Mouse Brain Tissue Extract
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Mn SOD polyclonal antibody Flow Cytometry Mn SOD polyclonal antibody Immunohistochemistry Mn SOD polyclonal antibody Western blot

Product Literature References

Daily blueberry consumption for 12 weeks improves endothelial function in postmenopausal women with above-normal blood pressure through reductions in oxidative stress: a randomized controlled trial: E.K. Woolf, et al.; Food Funct. 14, 2621 (2023), Abstract;
Immunomodulatory Function of Polyvinylpyrrolidone (PVP)-Functionalized Gold Nanoparticles in Vibrio-Stimulated Sea Urchin Immune Cells: A. Alijagic, et al.; Nanomaterials 11, 2646 (2021), Abstract;
Long-term voluntary running prevents the onset of symptomatic Friedreich's ataxia in mice: H. Zhao, et al.; Sci. Rep. 10, 6095 (2020), Abstract; Full Text
Placental Mitochondria Therapy for Cerebral Ischemia-Reperfusion Injury in Mice: Y. Nakamura, et al.; Stroke 51, 3142 (2020), Application(s): WB; mouse placenta, Abstract;
Interval running training improves age-related skeletal muscle wasting and bone loss: Experiments with ovariectomized rats: J.S. Kim, et al.; Exp. Physiol. 104, 691 (2019), Application(s): WB of rat muscle lystates, Abstract;
Levels of the Autophagy-Related 5 Protein Affect Progression and Metastasis of Pancreatic Tumors in Mice: K. Gorgulu, et al.; Gastroenterology 156, 203 (2019), Abstract;
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Bid maintains mitochondrial cristae structure and function and protects against cardiac disease in an integrative genomics study: C.T. Salisbury-Ruf, et al.; Elife 7, e40907 (2018), Abstract; Full Text
Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells: J. Geiger, et al.; Methods Mol. Biol. 1782, 337 (2018), Abstract;
Mitohormesis in Mice via Sustained Basal Activation of Mitochondrial and Antioxidant Signaling: C.S. Cox, et al.; Cell Metab. 28, 776 (2018), Abstract;
Protein Kinase C Epsilon Is a Key Regulator of Mitochondrial Redox Homeostasis in Acute Myeloid Leukemia: D. Di Marcantonio, et al.; Clin. Cancer Res. 24, 608 (2018), Abstract; Full Text
Angiotensin receptor blockade improves cardiac mitochondrial activity in response to an acute glucose load in obese insulin resistant rats: M. Thorwald, et al.; Redox Biol. 14, 371 (2017), Abstract; Full Text
Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors: N.J. Yates, et al.; Neural Regen. Res. 12, 307 (2017), Abstract; Full Text
Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes: M. Gomez-Serrano, et al.; Redox Biol. 11, 415 (2017), Abstract; Full Text
eIF2α phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet: W.G. Choi, et al.; Nutr. 14, 48 (2017), Abstract; Full Text
Oncogenic MCT-1 activation promotes YY1-EGFR-MnSOD signaling and tumor progression: H.Y. Tseng, et al.; Oncogenesis 6, e313 (2017), Abstract; Full Text
Mitochondrial response to the BCKDK-deficiency: Some clues to understand the positive dietary response in this form of autism: A. Oyarzabal, et al.; Biochim. Biophys. Acta 1862, 592 (2016), Abstract;
Analysis of the Behavior of Mitochondria in the Ovaries of the Earthworm Dendrobaena veneta Rosa 1839: J. Faron, et al.; PLoS One 10, e0117187 (2015), Application(s): Immunofluorescence, Abstract; Full Text
Brain-Specific Superoxide Dismutase 2 Deficiency Causes Perinatal Death with Spongiform Encephalopathy in Mice: N. Izuo, et al.; Oxid. Med. Cell. Longev. 2015, Article ID 238914 (2015), Application(s): Western Blotting, Abstract; Full Text
NR2B-dependent cyclophilin D translocation suppresses the recovery of synaptic transmission after oxygen-glucose deprivation: Z. Zhang, et al.; Biochim. Biophys. Acta 1852, 2225 (2015), Application(s): Western blot , Abstract;
Resveratrol and N-acetylcysteine influence redox balance in equine articular chondrocytes under acidic and very low oxygen conditions: J.A. Collins, et al.; Free Radic. Biol. Med. 86, 57 (2015), Application(s): Western Blot, Abstract;
Scheme of Ischaemia-triggered Agents during Brain Infarct Evolution in a Rat Model of Permanent Focal Ischaemia: P. Bonova, et al.; J. Mol. Neurosci. 57, 73 (2015), Application(s): Western Blot, Abstract;
Autophagy and oxidative stress in gliomas with IDH1 mutations: M.R. Gilbert, et al.; Acta Neuropathol. 127, 221 (2014), Abstract; Full Text
Essential role of protein-tyrosine phosphatase 1B in the modulation of insulin signaling by acetaminophen in hepatocytes: M.A. Mobasher, et al.; J. Biol. Chem. 289, 29406 (2014), Abstract; Full Text
S-allyl cysteine protects against MPTP-induced striatal and nigral oxidative neurotoxicity in mice: participation of Nrf2: E. Garcia, et al.; Free Radic. Res. 48, 159 (2014), Abstract;
Functional characterization of novel genotypes and cellular oxidative stress studies in propionic acidemia: L. Gallego-Villar, et al.; J. Inherit. Metab. Dis. 36, 731 (2013), Abstract;
Zymogram profiling of superoxide dismutase and catalase activities allows Saccharomyces and non-Saccharomyces species differentiation and correlates to their fermentation performance: E. Gamero-Sandemetrio, et al.; Appl. Microbiol. Biotechnol. 97, 4563 (2013), Abstract;
Activation of PPARδ prevents endothelial dysfunction induced by overexpression of amyloid-β precursor protein: L.V. d'Uscio, et al.; Cardiovasc. Res. 96, 504 (2012), Application(s): Western Blot of mouse aorta lysates, Abstract; Full Text
Effect of exposure to benzo[a]pyrene on SODs, CYP1A1/1A2- and CYP2E1 immunopositive proteins in the blood clam Scapharca inaequivalvis: M. Monari, et al. ; Mar. Environ. Res. 63, 200 (2007), Application(s): WB using clam tissue, Abstract;
Model mice for tissue-specific deletion of the manganese superoxide dismutase (MnSOD) gene: T. Shirasawa, et al. ; Biochem. Biophys. Res. Commun. 296, 729 (2002), Application(s): WB using mouse samples, Abstract;
Superoxide dismutase activity in organotypic midbrain-striatum co-cultures is associated with resistance of dopaminergic neurons to excitotoxicity: A. Akaike, et al. ; J. Neurochem. 76, 1336 (2001), Application(s): WB using rat samples, Abstract;
Garlic ameliorates gentamicin nephrotoxicity: relation to antioxidant enzymes: M.E. Ibarra-Rubio, et al. ; Free Radic. Biol. Med. 29, 602 (2000), Application(s): WB using rat samples, Abstract;

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