Product Details
Alternative Name: | GFP |
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Clone: | 1E4 |
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Host: | Mouse |
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Isotype: | IgG2b |
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Immunogen: | Recombinant GFP protein (Aequorea victoria). |
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UniProt ID: | P42212 |
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Gene/Protein Identifier: | JQ1514 (PIR) |
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Species reactivity: | Species independent
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Applications: | ICC, IHC, IP, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (2µg/ml) Immunohistochemistry (10µg/ml) Immunoprecipitation (5µg/ml) Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~27kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The green fluorescent protein (GFP) is derived from the jellyfish Aequorea victoria, that fluoresces green when exposed to blue light. GFP is a frequently used reporter for gene expression in recombinant DNA and cell transfection systems. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Myofibril growth during cardiac hypertrophy is regulated through dual phosphorylation and acetylation of the actin capping protein CapZ: Y.H. Lin, et al.; Cell. Signal.
28, 1015 (2016),
Application(s): Co-Immunoprecipitation,
Abstract;
Efficient cross-presentation depends on autophagy in tumor cells: H. Hu, et al. ; Cancer Res.
68, 6889 (2008),
Application(s): IP, WB using human cell lysates,
Abstract;