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Green Fluorescent Protein monoclonal antibody (1E4)

 
ADI-SAB-500-E 100 µg 304.00 USD
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Product Specification

Alternative Name:GFP
 
Clone:1E4
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant GFP protein (Aequorea victoria).
 
UniProt ID:P42212
 
Gene/Protein Identifier:JQ1514 (PIR)
 
Species reactivity:Species independent
 
Applications:ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (2µg/ml)
Immunohistochemistry (10µg/ml)
Immunoprecipitation (5µg/ml)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~27kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The green fluorescent protein (GFP) is derived from the jellyfish Aequorea victoria, that fluoresces green when exposed to blue light. GFP is a frequently used reporter for gene expression in recombinant DNA and cell transfection systems.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Myofibril growth during cardiac hypertrophy is regulated through dual phosphorylation and acetylation of the actin capping protein CapZ: Y.H. Lin, et al.; Cell. Signal. 28, 1015 (2016), Application(s): Co-Immunoprecipitation, Abstract;
Efficient cross-presentation depends on autophagy in tumor cells: H. Hu, et al. ; Cancer Res. 68, 6889 (2008), Application(s): IP, WB using human cell lysates, Abstract;

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