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FLAG polyclonal antibody

ADI-SAB-410-0100 100 µg 275.00 USD
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Product Details

Immunogen:Synthetic peptide (CDYKDDDDK) coupled to KLH.
Source:Purified from rabbit serum.
Species reactivity:Species independent
Specificity:Recognizes FLAG-tagged proteins.
Applications:ELISA, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Immunoaffinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:FLAG-tag stands for an octapeptide with a sequence of DYKDDDDK. It is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It is widely used for affinity chromatography to purify recombinant proteins from variety of systems. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence, such as in ELISA, Western Blot, etc.
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co. LLC
Regulatory Status:RUO - Research Use Only
FLAG polyclonal antibody Western blot
Western blot analysis of anti-FLAG, pAb (Prod. No. ADI-SAB-410): Lane 1: MW marker, Lane 2: SuperFasLigand™ (soluble) (human), (recombinant) (Prod. No. ALX-522-020), Lane 3: MW marker, Lane 4: SuperFasLigand™ (soluble) (human), (recombinant) (Prod. No. ALX-522-020).
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FLAG polyclonal antibody Western blot

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