Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human, mouse, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.
Product Details
Host: | Goat |
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Immunogen: | Purified rat IgG (whole molecule). |
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Species reactivity: | Rat
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Specificity: | Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. |
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Applications: | ELISA, ICC, IHC, WB
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Recommended Dilutions/Conditions: | ELISA (1:5,000-1:100,000) Immunocytochemistry (1:500-1:5,000) Immunohistochemistry(1:500-1:5,000) Western Blot (1:10,000-1:200,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Immunoaffinity purified. |
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Quality Control: | The antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with human, mouse, bovine, horse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species. |
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Formulation: | Liquid. In 0.005M sodium phosphate, pH 7.6, containing 0.125M sodium chloride, 7.5mg/ml BSA (IgG-free, protease-free), and 50% glycerol. |
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Use/Stability: | Warning that sodium azide as a preservative will substantially inhibit the enzyme activity of HRP. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Immunoglobulin G (IgG) is an immunoglobulin monomer consisting of two (gamma) heavy chains and two light chains. Each IgG molecule contains two antigen binding domains and a single effector (Fc) domain. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Systemic Mobilization of Breast Cancer Resistance Protein in Response to Oncogenic Stress: M. Szczygieł, et al.; Cancers
14, 313 (2022),
Abstract;
Exosomes influence the behavior of human mesenchymal stem cells on titanium surfaces: X. Wang, et al.; Biomaterials
230, 119571 (2020),
Application(s): WB,
Abstract;
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