Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Akt2 (human), (recombinant)

 
ADI-PPK-411-Z 5 µg 409.00 USD
Do you need bulk/larger quantities?
 

Product Details

Alternative Name:Protein kinase B β, PKBβ, RAC-β serine/threonine-protein kinase
 
Source:Produced in Sf9 insect cells. Produced in a baculovirus expression system.
 
UniProt ID:P31751
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 25% glycerol, 0.1mM EDTA, and 0.1mM PMSF.
 
Purity:≥75% (Densitometry)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:Kinase assay
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3β, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.
 
Regulatory Status:RUO - Research Use Only
 
Akt2 (human), (recombinant) SDS-PAGE
SDS-PAGE analysis of Akt2 (human), (recombinant).
Akt2 (human), (recombinant) Activity assay
Activity Assay
Please mouse over
Akt2 (human), (recombinant) SDS-PAGE Akt2 (human), (recombinant) Activity assay

Product Toolbox

PRODUCT RESOURCES

Datasheet
SDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Akt
By biological activity:
Akt Recombinant protein
Akt Enzyme
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Essential Research Tools for Neurodegeneration & Neural Signaling
Essential Research Tools for Neurodegeneration & Neural Signaling
Download as PDF

All new literature pieces