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p47Phox (phosphorylated) (human), (recombinant) (His-tag)

ADI-OSP-560-050 50 µg 259.00 USD
ADI-OSP-560-200 200 µg 556.00 USD
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Product Specification

Alternative Name:NCF1, Neutrophil cytosolic factor 1
Recommended Dilutions/Conditions:Western Blot (10ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Source:Produced in E. coli. Human phosphorylated p47Phox is fused at the N-terminus to a His-tag.
UniProt ID:P14598
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 150mM sodium chloride and 1mM DTT.
Purity:≥95% (SDS-PAGE; Western blot)
Purity Detail:Purified by multi-step chromatography.
Endotoxin Content:<50EU/mg purified protein (LAL test)
Specificity:This protein is immuno-reactive with antibodies raised against pSer304 and fails to react with antibodies raised against pSer359.
Application Notes:Western blot control.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Scientific Background:Originally recognized for their role in the respiratory burst of phagocytes, the NOX family of NADPH oxidases is now known to consist of at least 13 enzymes and enzyme subunits that catalyze the production of superoxide and other downstream reactive oxygen species (ROS) in nearly all cells and tissues. NOX enzymes contribute to a multitude of physiological functions including host defense, cell signaling, and differentiation, with dysregulation linked to pathological manifestations including immunosuppression, hypothyroidism, cardiovascular disease, and neurodegeneration. p47Phox, also known as neutrophil cytosolic factor 1 (NCF1), is considered the organizing subunit of NADPH oxidase enzyme complexes containing the prototypic NOX2 enzyme. Cell stimulation results in phosphorylation of p47Phox, exposing a proline rich region which mediates translocation and association with membrane-associated NOX2/p22 complexes. Translocation of p47Phox is accompanied by the p40Phox subunit and p67Phox activator subunit, which along with GTP-bound Rac completes formation of the active NOX2 oxidase complex. Defects in p47Phox are associated with chronic granulomatous disease (CGD), characterized by an inability of host macrophages and phagocytes to kill ingested microbes, leading to life-threatening infections.
Regulatory Status:RUO - Research Use Only
p47Phox (phosphorylated) (human), (recombinant) (His-tag) Western blot
Western blot analysis: Lane 1: MW marker, Lane 2: p47Phox, Lane 3: p47Phox (phospho). Left Blot probed with p47Phox mAb. Right Blot probed with p47Phox (pSer304) pAb.
p47Phox (phosphorylated) (human), (recombinant) (His-tag) SDS-PAGE
SDS-PAGE analysis: Lane 1: MWM, Lane 2: 1µg, Lane 3: 2µg, Lane 4: 5µg p47Phox (phospho).
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p47Phox (phosphorylated) (human), (recombinant) (His-tag) Western blot p47Phox (phosphorylated) (human), (recombinant) (His-tag) SDS-PAGE

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p47Phox (human), (recombinant) (His-tag) 

Produced in E. coli. Human p47Phox is fused at the N-terminus to a His-tag., ≥95% (SDS-PAGE; Western blot), WB | Print as PDF
ADI-OSP-550-050 50 µg 250.00 USD
ADI-OSP-550-200 200 µg 488.00 USD
Do you need bulk/larger quantities?

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