Alternative Name: | NEFH, NF-H |
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Host: | Rabbit |
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Immunogen: | Native bovine Neurofilament NF-H. |
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UniProt ID: | P12036 (human) |
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Species reactivity: | Human, Mouse, Rat
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Applications: | ICC, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (1:100-1:500) Western Blot (1:1,000-1:2500) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~200-220kDa by Western blot. |
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Formulation: | Liquid. Whole rabbit antiserum containing 50% glycerol, 0.01% sodium azide, and 1.0mg/ml BSA. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Neuron-specific neurofilaments belong to the intermediate filament protein family, also known as 10nm filaments. As major constituents of the axonal cytoskeleton, neurofilaments contain heteropolymers of three major proteins: 200 kDa heavy molecular weight protein NF-H, 160 kDa medium molecular weight protein NF-M, and 68 kDa light molecular weight protein NF-L, with the ratio of each changing during neuronal development. All three proteins contain globular head and tail domains flanking a central alpha-helical domain. An accumulation of neurofilament proteins and/or mutations in one of the three major proteins occur during the progression of numerous neurological diseases, including ALS. |
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Regulatory Status: | RUO - Research Use Only |
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