| Alternative Name: | NEFH, NF-H |
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| Host: | Rabbit |
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| Immunogen: | Native bovine Neurofilament NF-H. |
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| UniProt ID: | P12036 (human) |
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| Species reactivity: | Human, Mouse, Rat
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| Applications: | ICC, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:100-1:500)
Western Blot (1:1,000-1:2500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~200-220kDa by Western blot. |
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| Formulation: | Liquid. Whole rabbit antiserum containing 50% glycerol, 0.01% sodium azide, and 1.0mg/ml BSA. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Neuron-specific neurofilaments belong to the intermediate filament protein family, also known as 10nm filaments. As major constituents of the axonal cytoskeleton, neurofilaments contain heteropolymers of three major proteins: 200 kDa heavy molecular weight protein NF-H, 160 kDa medium molecular weight protein NF-M, and 68 kDa light molecular weight protein NF-L, with the ratio of each changing during neuronal development. All three proteins contain globular head and tail domains flanking a central alpha-helical domain. An accumulation of neurofilament proteins and/or mutations in one of the three major proteins occur during the progression of numerous neurological diseases, including ALS. |
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| Regulatory Status: | RUO - Research Use Only |
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